CRISPR/Cas13a-assisted AMP generation for SARS-CoV-2 RNA detection using a personal glucose meter

CRISPR/Cas13a-assisted AMP generation for SARS-CoV-2 RNA detection using a personal glucose meter

Herein, we described a washing- and label-free clustered regularly interspaced short palindromic repeats (CRISPR)/LwaCas13a-based RNA detection method utilizing a personal glucose meter (PGM), which relies on the trans-cleavage activity of CRISPR/Cas13a and kinase reactions. In principle, the presen...

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Bibliographic Details
Main Authors: Junhyun Park, Hyogu Han, Jae Hoon Jeung, Hyowon Jang, Chihyun Park, Jun Ki Ahn
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Biosensors and Bioelectronics: X
Subjects:
SARS-CoV-2
CRISPR/Cas13a
Kinase
Cascade enzymatic reaction
Personal glucose meter
Biosensor
Online Access:http://www.sciencedirect.com/science/article/pii/S2590137022001765

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http://www.sciencedirect.com/science/article/pii/S2590137022001765

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