Pitfalls of improperly procured adjacent non-neoplastic tissue for somatic mutation analysis using next-generation sequencing

Pitfalls of improperly procured adjacent non-neoplastic tissue for somatic mutation analysis using next-generation sequencing

Abstract Background The rapid adoption of next-generation sequencing provides an efficient system for detecting somatic alterations in neoplasms. The detection of such alterations requires a matched non-neoplastic sample for adequate filtering of non-somatic events such as germline polymorphisms. No...

Full description

Bibliographic Details
Main Authors: Lei Wei, Antonios Papanicolau-Sengos, Song Liu, Jianmin Wang, Jeffrey M. Conroy, Sean T. Glenn, Elizabeth Brese, Qiang Hu, Kiersten Marie Miles, Blake Burgher, Maochun Qin, Karen Head, Angela R. Omilian, Wiam Bshara, John Krolewski, Donald L. Trump, Candace S. Johnson, Carl D. Morrison
Format: Article
Language:English
Published: BMC 2016-10-01
Series:BMC Medical Genomics
Subjects:
Somatic mutations
Tumor contamination
Adjacent normal tissues
Online Access:http://link.springer.com/article/10.1186/s12920-016-0226-1

Internet

http://link.springer.com/article/10.1186/s12920-016-0226-1

Similar Items