OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
Abstract Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructi...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-021-00779-0 |
| _version_ | 1818435157745467392 |
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| author | Zhiming Li Yan Zhang Xinzong Zhang Congcong Cao Xiaomin Luo Yaoting Gui Yunge Tang Shuiqiao Yuan |
| author_facet | Zhiming Li Yan Zhang Xinzong Zhang Congcong Cao Xiaomin Luo Yaoting Gui Yunge Tang Shuiqiao Yuan |
| author_sort | Zhiming Li |
| collection | DOAJ |
| description | Abstract Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99 %). Then, we examined the expression of OTOGL in developing mouse testes. Otogl mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global Otogl knockout mouse (KO) model using the CRISPR/Cas9 technology; however, Otogl KO mice displayed normal development and fertility. Further histological analysis of Otogl knockout mouse testes revealed that all types of spermatogenic cells are present in Otogl KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis. |
| first_indexed | 2024-12-14T16:48:25Z |
| format | Article |
| id | doaj.art-b6d0b63323664df2ae3747d9e4e5df19 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-14T16:48:25Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-b6d0b63323664df2ae3747d9e4e5df192022-12-21T22:54:07ZengBMCReproductive Biology and Endocrinology1477-78272021-06-011911810.1186/s12958-021-00779-0OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in miceZhiming Li0Yan Zhang1Xinzong Zhang2Congcong Cao3Xiaomin Luo4Yaoting Gui5Yunge Tang6Shuiqiao Yuan7Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyNHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong ProvinceInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University- Hong Kong University of Science and Technology Medical CenterGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University- Hong Kong University of Science and Technology Medical CenterNHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong ProvinceInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99 %). Then, we examined the expression of OTOGL in developing mouse testes. Otogl mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global Otogl knockout mouse (KO) model using the CRISPR/Cas9 technology; however, Otogl KO mice displayed normal development and fertility. Further histological analysis of Otogl knockout mouse testes revealed that all types of spermatogenic cells are present in Otogl KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis.https://doi.org/10.1186/s12958-021-00779-0OTOGLSpermatogenesisFertilityKnockout mouse modelCRISPR/Cas9 |
| spellingShingle | Zhiming Li Yan Zhang Xinzong Zhang Congcong Cao Xiaomin Luo Yaoting Gui Yunge Tang Shuiqiao Yuan OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice Reproductive Biology and Endocrinology OTOGL Spermatogenesis Fertility Knockout mouse model CRISPR/Cas9 |
| title | OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice |
| title_full | OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice |
| title_fullStr | OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice |
| title_full_unstemmed | OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice |
| title_short | OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice |
| title_sort | otogl a gelforming mucin protein is nonessential for male germ cell development and spermatogenesis in mice |
| topic | OTOGL Spermatogenesis Fertility Knockout mouse model CRISPR/Cas9 |
| url | https://doi.org/10.1186/s12958-021-00779-0 |
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