Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma
In colorectal cancer (CRC), the role of microsatellite instability (MSI) is well known. In a genome-wide scale, for the first time, we explored whether differential methylation is associated with MSI. We analyzed 250 paired samples from 125 CRC patients (m = 72, f = 53) at different stages. Of them,...
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MDPI AG
2021-10-01
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author | Farzana Jasmine Zahidul Haq Mohammed Kamal Maruf Raza Gustavo da Silva Katrina Gorospe Rupash Paul Patrick Strzempek Habibul Ahsan Muhammad G Kibriya |
author_facet | Farzana Jasmine Zahidul Haq Mohammed Kamal Maruf Raza Gustavo da Silva Katrina Gorospe Rupash Paul Patrick Strzempek Habibul Ahsan Muhammad G Kibriya |
author_sort | Farzana Jasmine |
collection | DOAJ |
description | In colorectal cancer (CRC), the role of microsatellite instability (MSI) is well known. In a genome-wide scale, for the first time, we explored whether differential methylation is associated with MSI. We analyzed 250 paired samples from 125 CRC patients (m = 72, f = 53) at different stages. Of them, 101 had left-sided CRC, 30 had MSI, 34 had somatic mutation in KRAS proto-oncogene (<i>KRAS</i>), and 6 had B-Raf proto-oncogene (<i>BRAF</i>) exon 15p.V600E mutation. MSI was more frequent in right-sided tumors (54% vs. 17%, <i>p</i> = 0.003). Among the microsatellite stable (MSS) CRC, a paired comparison revealed 1641 differentially methylated loci (DML) covering 686 genes at FDR 0.001 with delta beta ≥ 20%. Similar analysis in MSI revealed 6209 DML covering 2316 genes. ANOVA model including interaction (Tumor*MSI) revealed 23,322 loci, where the delta beta was different among MSI and MSS patients. Our study shows an association between MSI and tumor DNA methylation in the pathogenesis of CRC. Given the interaction seen in this study, it may be worth considering the MSI status while looking for methylation markers in CRC. The study also indicates an opportunity for potential use of certain immune checkpoint inhibitors (<i>CTLA4</i> and <i>HAVCR2</i> inhibitors) in CRC with MSI. |
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series | Cancers |
spelling | doaj.art-b70b09f5c81c484d95adc3dba6a766972023-11-22T15:54:52ZengMDPI AGCancers2072-66942021-10-011319495610.3390/cancers13194956Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal CarcinomaFarzana Jasmine0Zahidul Haq1Mohammed Kamal2Maruf Raza3Gustavo da Silva4Katrina Gorospe5Rupash Paul6Patrick Strzempek7Habibul Ahsan8Muhammad G Kibriya9Institute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USADepartment of Surgery, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, BangladeshDepartment of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, BangladeshDepartment of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, BangladeshInstitute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USAInstitute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USADepartment of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, BangladeshInstitute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USAInstitute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USAInstitute for Population and Precision Health, Department of Public Health Sciences, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USAIn colorectal cancer (CRC), the role of microsatellite instability (MSI) is well known. In a genome-wide scale, for the first time, we explored whether differential methylation is associated with MSI. We analyzed 250 paired samples from 125 CRC patients (m = 72, f = 53) at different stages. Of them, 101 had left-sided CRC, 30 had MSI, 34 had somatic mutation in KRAS proto-oncogene (<i>KRAS</i>), and 6 had B-Raf proto-oncogene (<i>BRAF</i>) exon 15p.V600E mutation. MSI was more frequent in right-sided tumors (54% vs. 17%, <i>p</i> = 0.003). Among the microsatellite stable (MSS) CRC, a paired comparison revealed 1641 differentially methylated loci (DML) covering 686 genes at FDR 0.001 with delta beta ≥ 20%. Similar analysis in MSI revealed 6209 DML covering 2316 genes. ANOVA model including interaction (Tumor*MSI) revealed 23,322 loci, where the delta beta was different among MSI and MSS patients. Our study shows an association between MSI and tumor DNA methylation in the pathogenesis of CRC. Given the interaction seen in this study, it may be worth considering the MSI status while looking for methylation markers in CRC. The study also indicates an opportunity for potential use of certain immune checkpoint inhibitors (<i>CTLA4</i> and <i>HAVCR2</i> inhibitors) in CRC with MSI.https://www.mdpi.com/2072-6694/13/19/4956MSIcolorectal cancerinteractionCIMPMMRimmune checkpoint inhibitor |
spellingShingle | Farzana Jasmine Zahidul Haq Mohammed Kamal Maruf Raza Gustavo da Silva Katrina Gorospe Rupash Paul Patrick Strzempek Habibul Ahsan Muhammad G Kibriya Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma Cancers MSI colorectal cancer interaction CIMP MMR immune checkpoint inhibitor |
title | Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma |
title_full | Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma |
title_fullStr | Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma |
title_full_unstemmed | Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma |
title_short | Interaction between Microsatellite Instability (MSI) and Tumor DNA Methylation in the Pathogenesis of Colorectal Carcinoma |
title_sort | interaction between microsatellite instability msi and tumor dna methylation in the pathogenesis of colorectal carcinoma |
topic | MSI colorectal cancer interaction CIMP MMR immune checkpoint inhibitor |
url | https://www.mdpi.com/2072-6694/13/19/4956 |
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