Strategy for chromosomal gene targeting in RecA-deficient Escherichia coli strains
Reengineering DNA by homologous recombination in Escherichia coli often depends on helper functions provided on a temporarily introduced replicon that is subsequently cured from the cells. The suicide vector pKSS offers a new curing strategy. pKSS specifies a variant of phenylalanyl-transfer RNA (tR...
| Main Authors: | , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
Taylor & Francis Group
2005-03-01
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| 叢編: | BioTechniques |
| 在線閱讀: | https://www.future-science.com/doi/10.2144/05383ST03 |