Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

Summary: Genome-edited human pluripotent stem cells (hPSCs) have broad applications in disease modeling, drug discovery, and regenerative medicine. We present and characterize a robust method for rapid, scarless introduction or correction of disease-associated variants in hPSCs using CRISPR/Cas9. Ut...

Full description

Bibliographic Details
Main Authors: Benjamin Steyer, Qian Bu, Evan Cory, Keer Jiang, Stella Duong, Divya Sinha, Stephanie Steltzer, David Gamm, Qiang Chang, Krishanu Saha
Format: Article
Language:English
Published: Elsevier 2018-02-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117305520

Internet

http://www.sciencedirect.com/science/article/pii/S2213671117305520

Similar Items