Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture

Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture

Summary: Here, we provide a protocol combing single-cell genomics and functional experiments to study stepwise folliculogenesis. This protocol isolates different developmental stages of mouse ovarian follicles to perform single-cell RNA-seq and in vitro culture, allowing to dissect and validate key...

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Bibliographic Details
Main Authors: Jingjing Qian, Ran Zhu, Rui Yan, Xin Long, Fan Guo
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Developmental biology
Model Organisms
Molecular Biology
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004178

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http://www.sciencedirect.com/science/article/pii/S2666166722004178

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