Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max)
The soybean is widely used in food industry due to its nutritional and functional qualities, but some studies have pointed it as a potent food allergen, especially in children. The concentration of IgG, IgG1 and IgE immunoglobulins in mice supplemented orally with common and transgenic soy was analy...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1615038 |
| _version_ | 1819327139539845120 |
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| author | Marcelo Oliveira Holanda Bárbara Cerqueira Fiorio José Ytalo Gomes da Silva Chayane Gomes Marques Sandra Machado Lira Carla Laíne Silva Lima Joana Talita Galdino Costa Thais Vital de Freitas Lucelina Araújo da Silva Danielle Ferreira de Oliveira Claisa Andréa Freitas Rabelo Maria Izabel Florindo Guedes |
| author_facet | Marcelo Oliveira Holanda Bárbara Cerqueira Fiorio José Ytalo Gomes da Silva Chayane Gomes Marques Sandra Machado Lira Carla Laíne Silva Lima Joana Talita Galdino Costa Thais Vital de Freitas Lucelina Araújo da Silva Danielle Ferreira de Oliveira Claisa Andréa Freitas Rabelo Maria Izabel Florindo Guedes |
| author_sort | Marcelo Oliveira Holanda |
| collection | DOAJ |
| description | The soybean is widely used in food industry due to its nutritional and functional qualities, but some studies have pointed it as a potent food allergen, especially in children. The concentration of IgG, IgG1 and IgE immunoglobulins in mice supplemented orally with common and transgenic soy was analysed. Mice were divided into two groups (6 animals each): lyophilized conventional soy (LCS) and lyophilized transgenic soy (LTS). Both received 100 μg of extracts for 10 consecutive days. Animals before immunization (pre-immune) formed the control group. The serum samples of the animals were undergone enzyme-linked immunosorbent assay (ELISA). The results of the ELISA tests showed that LCS and LTS induced the synthesis of specific IgG1 and IgG classes immunoglobulins and induced the formation of IgE immunoglobulin when evaluated by western blotting test. These results evidence the allergenicity of soybean and individuals with some food hypersensibility should avoid the consumption of this food. |
| first_indexed | 2024-12-24T13:06:06Z |
| format | Article |
| id | doaj.art-b73ff823a1b9490c986778e642ee0f36 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-24T13:06:06Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-b73ff823a1b9490c986778e642ee0f362022-12-21T16:54:01ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130169270010.1080/09540105.2019.16150381615038Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max)Marcelo Oliveira Holanda0Bárbara Cerqueira Fiorio1José Ytalo Gomes da Silva2Chayane Gomes Marques3Sandra Machado Lira4Carla Laíne Silva Lima5Joana Talita Galdino Costa6Thais Vital de Freitas7Lucelina Araújo da Silva8Danielle Ferreira de Oliveira9Claisa Andréa Freitas Rabelo10Maria Izabel Florindo Guedes11Biotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryBiotechnology and Molecular Biology LaboratoryThe soybean is widely used in food industry due to its nutritional and functional qualities, but some studies have pointed it as a potent food allergen, especially in children. The concentration of IgG, IgG1 and IgE immunoglobulins in mice supplemented orally with common and transgenic soy was analysed. Mice were divided into two groups (6 animals each): lyophilized conventional soy (LCS) and lyophilized transgenic soy (LTS). Both received 100 μg of extracts for 10 consecutive days. Animals before immunization (pre-immune) formed the control group. The serum samples of the animals were undergone enzyme-linked immunosorbent assay (ELISA). The results of the ELISA tests showed that LCS and LTS induced the synthesis of specific IgG1 and IgG classes immunoglobulins and induced the formation of IgE immunoglobulin when evaluated by western blotting test. These results evidence the allergenicity of soybean and individuals with some food hypersensibility should avoid the consumption of this food.http://dx.doi.org/10.1080/09540105.2019.1615038conventional soytransgenic soyimmunoglobulins |
| spellingShingle | Marcelo Oliveira Holanda Bárbara Cerqueira Fiorio José Ytalo Gomes da Silva Chayane Gomes Marques Sandra Machado Lira Carla Laíne Silva Lima Joana Talita Galdino Costa Thais Vital de Freitas Lucelina Araújo da Silva Danielle Ferreira de Oliveira Claisa Andréa Freitas Rabelo Maria Izabel Florindo Guedes Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) Food and Agricultural Immunology conventional soy transgenic soy immunoglobulins |
| title | Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) |
| title_full | Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) |
| title_fullStr | Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) |
| title_full_unstemmed | Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) |
| title_short | Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max) |
| title_sort | humoral immune response in mice immunized orally with conventional and transgenic soy extracts glycine max |
| topic | conventional soy transgenic soy immunoglobulins |
| url | http://dx.doi.org/10.1080/09540105.2019.1615038 |
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