Tunable translation-level CRISPR interference by dCas13 and engineered gRNA in bacteria
Abstract Although CRISPR-dCas13, the RNA-guided RNA-binding protein, was recently exploited as a translation-level gene expression modulator, it has still been difficult to precisely control the level due to the lack of detailed characterization. Here, we develop a synthetic tunable translation-leve...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-49642-x |