Internal guide RNA interactions interfere with Cas9-mediated cleavage

Internal guide RNA interactions interfere with Cas9-mediated cleavage

While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.

Bibliographic Details
Main Authors: Summer B. Thyme, Laila Akhmetova, Tessa G. Montague, Eivind Valen, Alexander F. Schier
Format: Article
Language:English
Published: Nature Portfolio 2016-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms11750

Internet

https://doi.org/10.1038/ncomms11750

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