Internal guide RNA interactions interfere with Cas9-mediated cleavage
While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-06-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11750 |
| _version_ | 1818341829369659392 |
|---|---|
| author | Summer B. Thyme Laila Akhmetova Tessa G. Montague Eivind Valen Alexander F. Schier |
| author_facet | Summer B. Thyme Laila Akhmetova Tessa G. Montague Eivind Valen Alexander F. Schier |
| author_sort | Summer B. Thyme |
| collection | DOAJ |
| description | While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage. |
| first_indexed | 2024-12-13T16:05:01Z |
| format | Article |
| id | doaj.art-b7ba7609830548a29b3cdd09a5836f93 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-13T16:05:01Z |
| publishDate | 2016-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-b7ba7609830548a29b3cdd09a5836f932022-12-21T23:39:04ZengNature PortfolioNature Communications2041-17232016-06-01711710.1038/ncomms11750Internal guide RNA interactions interfere with Cas9-mediated cleavageSummer B. Thyme0Laila Akhmetova1Tessa G. Montague2Eivind Valen3Alexander F. Schier4Department of Molecular and Cellular Biology, Harvard UniversityDepartment of Molecular and Cellular Biology, Harvard UniversityDepartment of Molecular and Cellular Biology, Harvard UniversityDepartment of Informatics, Computational Biology Unit, University of BergenDepartment of Molecular and Cellular Biology, Harvard UniversityWhile the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.https://doi.org/10.1038/ncomms11750 |
| spellingShingle | Summer B. Thyme Laila Akhmetova Tessa G. Montague Eivind Valen Alexander F. Schier Internal guide RNA interactions interfere with Cas9-mediated cleavage Nature Communications |
| title | Internal guide RNA interactions interfere with Cas9-mediated cleavage |
| title_full | Internal guide RNA interactions interfere with Cas9-mediated cleavage |
| title_fullStr | Internal guide RNA interactions interfere with Cas9-mediated cleavage |
| title_full_unstemmed | Internal guide RNA interactions interfere with Cas9-mediated cleavage |
| title_short | Internal guide RNA interactions interfere with Cas9-mediated cleavage |
| title_sort | internal guide rna interactions interfere with cas9 mediated cleavage |
| url | https://doi.org/10.1038/ncomms11750 |
| work_keys_str_mv | AT summerbthyme internalguidernainteractionsinterferewithcas9mediatedcleavage AT lailaakhmetova internalguidernainteractionsinterferewithcas9mediatedcleavage AT tessagmontague internalguidernainteractionsinterferewithcas9mediatedcleavage AT eivindvalen internalguidernainteractionsinterferewithcas9mediatedcleavage AT alexanderfschier internalguidernainteractionsinterferewithcas9mediatedcleavage |