Manganese Ions Individually Alter the Reverse Transcription Signature of Modified Ribonucleosides

Manganese Ions Individually Alter the Reverse Transcription Signature of Modified Ribonucleosides

Reverse transcription of RNA templates containing modified ribonucleosides transfers modification-related information as misincorporations, arrest or nucleotide skipping events to the newly synthesized cDNA strand. The frequency and proportion of these events, merged from all sequenced cDNAs, yield...

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Bibliographic Details
Main Authors: Marco Kristen, Johanna Plehn, Virginie Marchand, Kristina Friedland, Yuri Motorin, Mark Helm, Stephan Werner
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Genes
Subjects:
reverse transcription
RT signature
RNA modifications
m<sup>1</sup>A
manganese chloride
Online Access:https://www.mdpi.com/2073-4425/11/8/950

Internet

https://www.mdpi.com/2073-4425/11/8/950

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