Optimization of The Electroporation Conditions for Transfection of Human Factor IX into The Goat Fetal Fibroblasts

Optimization of The Electroporation Conditions for Transfection of Human Factor IX into The Goat Fetal Fibroblasts

Objective: Electroporation is the most common method used for the transfection of DNA. Although this method has been attributed for various cell using different buffer compositions, the effects of DNA concentration on the transfection efficiency has not yet been studied. Here the correlation between...

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Bibliographic Details
Main Authors: Amir Amiri Yekta, Azam Dalman, Mohammad Hossein Sanati, Nayeralsadat Fatemi, Hamed Vazirinasab, Alireza Zomorodipour, Mohammad Chehrazi, Hamid Gourabi
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2013-01-01
Series:Cell Journal
Subjects:
Gene Transfer
Naked DNA
Electroporation
Transgenic Animals
Fibroblast
Online Access:http://celljournal.org/components4.php?rQV===AfAdzNApDZJRnblJXYw9lZ8BEMApDZJxWY0J3bQxWYuJXdvp2XmxHQxATNApDZJ52bpR3Yh9lZ

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http://celljournal.org/components4.php?rQV===AfAdzNApDZJRnblJXYw9lZ8BEMApDZJxWY0J3bQxWYuJXdvp2XmxHQxATNApDZJ52bpR3Yh9lZ

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