Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less than 1 h to complete.

Bibliographic Details
Main Authors: Roger S. Zou, Momcilo Gavrilov, Yang Liu, Dominique Rasoloson, Madison Conte, Justin Hardick, Leo Shen, Siqi Chen, Andrew Pekosz, Geraldine Seydoux, Yukari C. Manabe, Taekjip Ha
Format: Article
Language:English
Published: Nature Portfolio 2022-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-03242-x

Internet

https://doi.org/10.1038/s42003-022-03242-x

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