α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition
Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/580 |
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| author | Anasztázia Hetényi Enikő Szabó Norbert Imre Kaushik Nath Bhaumik Attila Tököli Tamás Füzesi Réka Hollandi Peter Horvath Ágnes Czibula Éva Monostori Mária A. Deli Tamás A. Martinek |
| author_facet | Anasztázia Hetényi Enikő Szabó Norbert Imre Kaushik Nath Bhaumik Attila Tököli Tamás Füzesi Réka Hollandi Peter Horvath Ágnes Czibula Éva Monostori Mária A. Deli Tamás A. Martinek |
| author_sort | Anasztázia Hetényi |
| collection | DOAJ |
| description | Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform. |
| first_indexed | 2024-03-09T12:59:07Z |
| format | Article |
| id | doaj.art-b7ffb04ba3d847a5a22677125d34c90c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:59:07Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-b7ffb04ba3d847a5a22677125d34c90c2023-11-30T21:56:56ZengMDPI AGPharmaceutics1999-49232022-03-0114358010.3390/pharmaceutics14030580α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside RecognitionAnasztázia Hetényi0Enikő Szabó1Norbert Imre2Kaushik Nath Bhaumik3Attila Tököli4Tamás Füzesi5Réka Hollandi6Peter Horvath7Ágnes Czibula8Éva Monostori9Mária A. Deli10Tamás A. Martinek11Department of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryInstitute of Genetics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryDepartment of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryDepartment of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryDepartment of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryDepartment of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryInstitute of Biophysics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryInstitute of Biophysics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryInstitute of Genetics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryInstitute of Genetics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungarySynthetic and Systems Biology Unit, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, HungaryDepartment of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, HungaryCell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform.https://www.mdpi.com/1999-4923/14/3/580cell deliveryglycan recognitionalpha-beta peptideendocytosisimmunoglobulin |
| spellingShingle | Anasztázia Hetényi Enikő Szabó Norbert Imre Kaushik Nath Bhaumik Attila Tököli Tamás Füzesi Réka Hollandi Peter Horvath Ágnes Czibula Éva Monostori Mária A. Deli Tamás A. Martinek α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition Pharmaceutics cell delivery glycan recognition alpha-beta peptide endocytosis immunoglobulin |
| title | α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition |
| title_full | α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition |
| title_fullStr | α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition |
| title_full_unstemmed | α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition |
| title_short | α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition |
| title_sort | α β peptides as nanomolar triggers of lipid raft mediated endocytosis through gm1 ganglioside recognition |
| topic | cell delivery glycan recognition alpha-beta peptide endocytosis immunoglobulin |
| url | https://www.mdpi.com/1999-4923/14/3/580 |
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