Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay

Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay

The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL3...

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Bibliographic Details
Main Authors: Leonardo Garcia Monte, Fernanda Munhoz Dos Anjos Leal, Daiane Drawanz Hartwig, Sílvio Arruda Vasconcellos, Bibiana Brihuega, Odir Antonio Dellagostin, Cláudia Pinho Hartleben
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2014-06-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Pichia pastoris
LipL32
ELISA
Leptospirosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007&lng=en&tlng=en

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