Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay

Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay

Abstract Background Bacillus subtilis is one of the workhorses in industrial biotechnology and well known for its secretion potential. Efficient secretion of recombinant proteins still requires extensive optimization campaigns and screening with activity-based methods. However, not every protein can...

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Bibliographic Details
Main Authors: Patrick Lenz, Patrick J. Bakkes, Carolin Müller, Marzena Malek, Roland Freudl, Marco Oldiges, Thomas Drepper, Karl-Erich Jaeger, Andreas Knapp
Format: Article
Language:English
Published: BMC 2023-10-01
Series:Microbial Cell Factories
Subjects:
Bacillus subtilis
Protein secretion
Split GFP assay
Secretion stress biosensor
Online detection
Online Access:https://doi.org/10.1186/s12934-023-02199-8

Internet

https://doi.org/10.1186/s12934-023-02199-8

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