Cell-Cycle-Regulated Interaction between Mcm10 and Double Hexameric Mcm2-7 Is Required for Helicase Splitting and Activation during S Phase

Cell-Cycle-Regulated Interaction between Mcm10 and Double Hexameric Mcm2-7 Is Required for Helicase Splitting and Activation during S Phase

Mcm2-7 helicase is loaded onto double-stranded origin DNA as an inactive double hexamer (DH) in G1 phase. The mechanisms of Mcm2-7 remodeling that trigger helicase activation in S phase remain unknown. Here, we develop an approach to detect and purify the endogenous DHs directly. Through cellular fr...

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Bibliographic Details
Main Authors: Yun Quan, Yisui Xia, Lu Liu, Jiamin Cui, Zhen Li, Qinhong Cao, Xiaojiang S. Chen, Judith L. Campbell, Huiqiang Lou
Format: Article
Language:English
Published: Elsevier 2015-12-01
Series:Cell Reports
Subjects:
DNA replication
cell cycle
helicase activation
Mcm2-7
Mcm10
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124715013182

Internet

http://www.sciencedirect.com/science/article/pii/S2211124715013182

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