Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1

Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1

A new protein immobilization and purification system has been developed based on the improved plasmid vectors, designated pETChBD-X, which contained the gene coding for two novel chitin-binding domains ChBD-AB, factor Xa cleavage site and adapted for gene fusions. The ChBD-AD from Chitinolyticbacter...

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Bibliographic Details
Main Authors: Jie Zhou, Jianhao Chen, Nisha Zhuang, Alei Zhang, Kequan Chen, Ning Xu, Fengxue Xin, Wenming Zhang, Weiliang Dong, Min Jiang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
chitin-binding domain
fusion tag
affinity chromatography
protein purification
protein immobilization
enzyme conversion
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00579/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.00579/full

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