Modification of <i>i</i>-GONAD Suitable for Production of Genome-Edited C57BL/6 Inbred Mouse Strain

Modification of <i>i</i>-GONAD Suitable for Production of Genome-Edited C57BL/6 Inbred Mouse Strain

Improved genome editing via oviductal nucleic acid delivery (<i>i</i>-GONAD) is a novel method for producing genome-edited mice in the absence of ex vivo handling of zygotes. <i>i</i>-GONAD involves the intraoviductal injection of clustered regularly interspaced short palindr...

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Bibliographic Details
Main Authors: Yukari Kobayashi, Takuya Aoshima, Ryota Ito, Ryota Shinmura, Masato Ohtsuka, Eri Akasaka, Masahiro Sato, Shuji Takabayashi
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Cells
Subjects:
low-dose PMSG
genome editing
<i>i</i>-GONAD
C57BL/6J
in vivo electroporation
CRISPR/Cas9
Online Access:https://www.mdpi.com/2073-4409/9/4/957

Internet

https://www.mdpi.com/2073-4409/9/4/957

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