Kinetic Analysis Misinterpretations Due to the Occurrence of Enzyme Inhibition by Reaction Product: Comparison between Initial Velocities and Reaction Time Course Methodologies

The Michaelis–Menten equation (MME) has been extensively used in biochemical reactions, but it is not appropriate when the reaction product inhibits the enzyme. Under these circumstances, each determined initial velocity, v<sub>0</sub>, is one experimental point that actually belongs to...

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Bibliographic Details
Main Authors: Joana M. C. Fernandes, Albino A. Dias, Rui M. F. Bezerra
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/12/1/102