Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i>
Agro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by <i>Streptomyces thermocarboxydus<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/2/287 |
| _version_ | 1797410513941954560 |
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| author | Thi Ngoc Tran Chien Thang Doan San-Lang Wang |
| author_facet | Thi Ngoc Tran Chien Thang Doan San-Lang Wang |
| author_sort | Thi Ngoc Tran |
| collection | DOAJ |
| description | Agro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by <i>Streptomyces thermocarboxydus</i> TKU045. The optimal media for xylanase production was 2% (<i>w/v</i>) WBP, 1.50% (<i>w/v</i>) KNO<sub>3</sub>, 0.05% (<i>w/v</i>) MgSO<sub>4</sub>, and 0.10% (<i>w/v</i>) K<sub>2</sub>HPO<sub>4</sub>, and the optimal culture conditions were 50 mL (in a 250 mL-volume Erlenmeyer flask), initial pH 9.0, 37 °C, 125 rpm, and 48 h. Accordingly, the highest xylanase activity was 6.393 ± 0.130 U/mL, 6.9-fold higher than that from un-optimized conditions. <i>S. thermocarboxydus</i> TKU045 secreted at least four xylanases with the molecular weights of >180, 36, 29, and 27 kDa when cultured on the WBP-containing medium. The enzyme cocktail produced by <i>S. thermocarboxydus</i> TKU045 was optimally active over a broad range of temperature and pH (40–70 °C and pH 5–8, respectively) and could hydrolyze birchwood xylan to produce xylobiose as the major product. The obtained xylose oligosaccharide (XOS) were investigated for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the growth effect of lactic acid bacteria. Finally, the solid waste from the WBP fermentation using <i>S. thermocarboxydus</i> TKU045 revealed the high adsorption of Congo red, Red 7, and Methyl blue. Thus, <i>S. thermocarboxydus</i> TKU045 could be a potential strain to utilize wheat bran to produce xylanases for XOS preparation and dye adsorbent. |
| first_indexed | 2024-03-09T04:31:09Z |
| format | Article |
| id | doaj.art-b8bde8ce5b4148918742ef957b208706 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T04:31:09Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b8bde8ce5b4148918742ef957b2087062023-12-03T13:35:37ZengMDPI AGPolymers2073-43602021-01-0113228710.3390/polym13020287Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i>Thi Ngoc Tran0Chien Thang Doan1San-Lang Wang2Doctoral Program in Applied Sciences, College of Science, Tamkang University, New Taipei City 25137, TaiwanFaculty of Natural Sciences and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanAgro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by <i>Streptomyces thermocarboxydus</i> TKU045. The optimal media for xylanase production was 2% (<i>w/v</i>) WBP, 1.50% (<i>w/v</i>) KNO<sub>3</sub>, 0.05% (<i>w/v</i>) MgSO<sub>4</sub>, and 0.10% (<i>w/v</i>) K<sub>2</sub>HPO<sub>4</sub>, and the optimal culture conditions were 50 mL (in a 250 mL-volume Erlenmeyer flask), initial pH 9.0, 37 °C, 125 rpm, and 48 h. Accordingly, the highest xylanase activity was 6.393 ± 0.130 U/mL, 6.9-fold higher than that from un-optimized conditions. <i>S. thermocarboxydus</i> TKU045 secreted at least four xylanases with the molecular weights of >180, 36, 29, and 27 kDa when cultured on the WBP-containing medium. The enzyme cocktail produced by <i>S. thermocarboxydus</i> TKU045 was optimally active over a broad range of temperature and pH (40–70 °C and pH 5–8, respectively) and could hydrolyze birchwood xylan to produce xylobiose as the major product. The obtained xylose oligosaccharide (XOS) were investigated for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the growth effect of lactic acid bacteria. Finally, the solid waste from the WBP fermentation using <i>S. thermocarboxydus</i> TKU045 revealed the high adsorption of Congo red, Red 7, and Methyl blue. Thus, <i>S. thermocarboxydus</i> TKU045 could be a potential strain to utilize wheat bran to produce xylanases for XOS preparation and dye adsorbent.https://www.mdpi.com/2073-4360/13/2/287agro-byproductsdye adsorptionantioxidantprebiotic<i>Streptomyces thermocarboxydus</i>xylanase |
| spellingShingle | Thi Ngoc Tran Chien Thang Doan San-Lang Wang Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> Polymers agro-byproducts dye adsorption antioxidant prebiotic <i>Streptomyces thermocarboxydus</i> xylanase |
| title | Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> |
| title_full | Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> |
| title_fullStr | Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> |
| title_full_unstemmed | Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> |
| title_short | Conversion of Wheat Bran to Xylanases and Dye Adsorbent by <i>Streptomyces thermocarboxydus</i> |
| title_sort | conversion of wheat bran to xylanases and dye adsorbent by i streptomyces thermocarboxydus i |
| topic | agro-byproducts dye adsorption antioxidant prebiotic <i>Streptomyces thermocarboxydus</i> xylanase |
| url | https://www.mdpi.com/2073-4360/13/2/287 |
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