Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis...

Full description

Bibliographic Details
Main Authors: Raquel de A Maschmann, Mirela Verza, Marcia SN Silva, Rosa Dea Sperhacke, Marta O Ribeiro, Philip Noel Suffys, Harrison Magdinier Gomes, Enrico Tortoli, Fiorella Marcelli, Arnaldo Zaha, Maria Lucia R Rossetti
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2011-03-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
drug resistance
reverse dot blot hybridization
rifampin
rpoB
tuberculosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200004&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200004&lng=en&tlng=en

Similar Items