Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
<p>Abstract</p> <p>Background</p> <p>Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in cert...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2012-06-01
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Series: | BMC Bioinformatics |
Online Access: | http://www.biomedcentral.com/1471-2105/13/134 |