Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

<p>Abstract</p> <p>Background</p> <p>Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in cert...

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Bibliographic Details
Main Authors:	Ye Jian, Coulouris George, Zaretskaya Irena, Cutcutache Ioana, Rozen Steve, Madden Thomas L
Format:	Article
Language:	English
Published:	BMC 2012-06-01
Series:	BMC Bioinformatics
Online Access:	http://www.biomedcentral.com/1471-2105/13/134

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