Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency
The work describes the carotenoid synthesis process by <i>Rhodotorula glutinis</i> P4M422 using an agro-industrial waste as the substrate, seeking a biorefinery platform approach for waste utilization to produce high-value molecules. A culture medium based on goat milk whey (GMW) was opt...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/3/658 |
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| author | Luis Carlos Mata-Gómez Paula Mapelli-Brahm Antonio J. Meléndez-Martínez Alejandro Méndez-Zavala Lourdes Morales-Oyervides Julio Montañez |
| author_facet | Luis Carlos Mata-Gómez Paula Mapelli-Brahm Antonio J. Meléndez-Martínez Alejandro Méndez-Zavala Lourdes Morales-Oyervides Julio Montañez |
| author_sort | Luis Carlos Mata-Gómez |
| collection | DOAJ |
| description | The work describes the carotenoid synthesis process by <i>Rhodotorula glutinis</i> P4M422 using an agro-industrial waste as the substrate, seeking a biorefinery platform approach for waste utilization to produce high-value molecules. A culture medium based on goat milk whey (GMW) was optimized via the Taguchi method (L9 array). Four factors (ethanol, carbon and nitrogen source, and pH) were evaluated at three levels. The carbon and nitrogen composition were the factors dominating the process performance. Optimized conditions were validated (Urea, 0.3% <i>w</i>/<i>v</i>; pH, 4.5; ethanol, 10% <i>v</i>/<i>v</i>; glucose, 6.0%), and the carotenoid production (4075 µg/L) was almost 200% higher than when using the un-optimized process (2058 µg/L). Provitamin A carotenoids torulene, β–carotene, and γ–carotene (different proportions) were produced under all conditions. The hydrolyzed goat milk whey showed promising expectations as a low-cost source for carotenoid production by <i>Rhodotorula glutinis</i> P4M422. The results are important for the innovative sustainable production of carotenoid-rich matrices for different purposes (nutrition, health promotion, color) and industries (foods, nutricosmetics, nutraceuticals, feeds), notably to help to combat vitamin A deficiency. |
| first_indexed | 2024-03-11T09:44:14Z |
| format | Article |
| id | doaj.art-b8ec553af3b64d158c0daa0d4dd841b4 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T09:44:14Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b8ec553af3b64d158c0daa0d4dd841b42023-11-16T16:42:28ZengMDPI AGFoods2304-81582023-02-0112365810.3390/foods12030658Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A DeficiencyLuis Carlos Mata-Gómez0Paula Mapelli-Brahm1Antonio J. Meléndez-Martínez2Alejandro Méndez-Zavala3Lourdes Morales-Oyervides4Julio Montañez5Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, Coahuila, MexicoFood Colour and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainFood Colour and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainFacultad de Ciencias Quimicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, Coahuila, MexicoFacultad de Ciencias Quimicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, Coahuila, MexicoFacultad de Ciencias Quimicas, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 25280, Coahuila, MexicoThe work describes the carotenoid synthesis process by <i>Rhodotorula glutinis</i> P4M422 using an agro-industrial waste as the substrate, seeking a biorefinery platform approach for waste utilization to produce high-value molecules. A culture medium based on goat milk whey (GMW) was optimized via the Taguchi method (L9 array). Four factors (ethanol, carbon and nitrogen source, and pH) were evaluated at three levels. The carbon and nitrogen composition were the factors dominating the process performance. Optimized conditions were validated (Urea, 0.3% <i>w</i>/<i>v</i>; pH, 4.5; ethanol, 10% <i>v</i>/<i>v</i>; glucose, 6.0%), and the carotenoid production (4075 µg/L) was almost 200% higher than when using the un-optimized process (2058 µg/L). Provitamin A carotenoids torulene, β–carotene, and γ–carotene (different proportions) were produced under all conditions. The hydrolyzed goat milk whey showed promising expectations as a low-cost source for carotenoid production by <i>Rhodotorula glutinis</i> P4M422. The results are important for the innovative sustainable production of carotenoid-rich matrices for different purposes (nutrition, health promotion, color) and industries (foods, nutricosmetics, nutraceuticals, feeds), notably to help to combat vitamin A deficiency.https://www.mdpi.com/2304-8158/12/3/658Taguchibiotechnologycarotenoidsyeastprovitamin Asustainability |
| spellingShingle | Luis Carlos Mata-Gómez Paula Mapelli-Brahm Antonio J. Meléndez-Martínez Alejandro Méndez-Zavala Lourdes Morales-Oyervides Julio Montañez Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency Foods Taguchi biotechnology carotenoids yeast provitamin A sustainability |
| title | Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency |
| title_full | Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency |
| title_fullStr | Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency |
| title_full_unstemmed | Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency |
| title_short | Microbial Carotenoid Synthesis Optimization in Goat Cheese Whey Using the Robust Taguchi Method: A Sustainable Approach to Help Tackle Vitamin A Deficiency |
| title_sort | microbial carotenoid synthesis optimization in goat cheese whey using the robust taguchi method a sustainable approach to help tackle vitamin a deficiency |
| topic | Taguchi biotechnology carotenoids yeast provitamin A sustainability |
| url | https://www.mdpi.com/2304-8158/12/3/658 |
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