Simultaneous Detection of Multiple Point Mutations Using Fluorescence-Coupled Competitive Primer Extension
We report the development of a method for the simultaneous genotyping of several distinct nucleotide positions by means of fluorescence-coupled competitive primer extension. We demonstrate the application of this method for the simultaneous detection of three point mutations in the human mitochondri...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97225rr05 |