Development of a Versatile, Near Full Genome Amplification and Sequencing Approach for a Broad Variety of HIV-1 Group M Variants

Development of a Versatile, Near Full Genome Amplification and Sequencing Approach for a Broad Variety of HIV-1 Group M Variants

Near full genome sequencing (NFGS) of HIV-1 is required to assess the genetic composition of HIV-1 strains comprehensively. Population-wide, it enables a determination of the heterogeneity of HIV-1 and the emergence of novel/recombinant strains, while for each individual it constitutes a diagnostic...

Full description

Bibliographic Details
Main Authors:	Andrew N. Banin, Michael Tuen, Jude S. Bimela, Marcel Tongo, Paul Zappile, Alireza Khodadadi-Jamayran, Aubin J. Nanfack, Josephine Meli, Xiaohong Wang, Dora Mbanya, Jeanne Ngogang, Adriana Heguy, Phillipe N. Nyambi, Charles Fokunang, Ralf Duerr
Format:	Article
Language:	English
Published:	MDPI AG 2019-04-01
Series:	Viruses
Subjects:	Near full genome amplification and sequencing HIV-1 group M subtype-independent approach rational primer design single-genome amplification (SGA) third-generation sequencing (TGS) bulk sequencing and cloning
Online Access:	https://www.mdpi.com/1999-4915/11/4/317

Similar Items

Human Alphacoronavirus Universal Primers for Genome Amplification and Sequencing
by: Sungmi Choi, et al.
Published: (2022-03-01)

The use of RT-PCR in the diagnosis and differentiation of vaccine strains of chicken infectious bronchitis and Newcastle disease
by: Nigmetulla Assanov, et al.
Published: (2023-06-01)

Characterization of EIAV <i>env</i> Quasispecies during Long-Term Passage In Vitro: Gradual Loss of Pathogenicity
by: Cong Liu, et al.
Published: (2019-04-01)

Chimera: The spoiler in multiple displacement amplification
by: Na Lu, et al.
Published: (2023-01-01)

Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications
by: Shonan Sho, et al.
Published: (2017-07-01)

A multiplex primer design algorithm for target amplification of continuous genomic regions
by: Ahmet Rasit Ozturk, et al.
Published: (2017-06-01)

Benchmarking of Nanopore R10.4 and R9.4.1 flow cells in single-cell whole-genome amplification and whole-genome shotgun sequencing
by: Ying Ni, et al.
Published: (2023-01-01)

Technological considerations for genome-guided diagnosis and management of cancer
by: Niall J. Lennon, et al.
Published: (2016-10-01)

Applications of single-cell sequencing for human lung cancer: the progress and the future perspective
by: Min Zhang, et al.
Published: (2017-03-01)

SCCNV: A Software Tool for Identifying Copy Number Variation From Single-Cell Whole-Genome Sequencing
by: Xiao Dong, et al.
Published: (2020-11-01)

Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples
by: Zalak Shah, et al.
Published: (2020-03-01)

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia
by: Syahzuwan Hassan, et al.
Published: (2023-01-01)

Characterization of trypanosomes in N’Dama cattle of Mushie ranch in the Democratic Republic of the Congo
by: Edouard Telamanu BAFWANGA, et al.
Published: (2019-06-01)

Whole Genome Amplification in Preimplantation Genetic Testing in the Era of Massively Parallel Sequencing
by: Ludmila Volozonoka, et al.
Published: (2022-04-01)

Analyzing Modern Biomolecules: The Revolution of Nucleic-Acid Sequencing – Review
by: Gabriel Dorado, et al.
Published: (2021-07-01)

Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
by: Nicolas Berthet, et al.

Single-cell sequencing of the small and AT-skewed genome of malaria parasites
by: Shiwei Liu, et al.
Published: (2021-05-01)

Difference of molecular alterations in HER2-positive and HER2-negative gastric cancers by whole-genome sequencing analysis
by: Zhou C, et al.
Published: (2018-09-01)

Characterisation of novel endogenous geminiviral elements in macadamia
by: Mohamed C. M. Zakeel, et al.
Published: (2021-11-01)

A Rapid Drug Resistance Genotyping Workflow for Mycobacterium tuberculosis, Using Targeted Isothermal Amplification and Nanopore Sequencing
by: Harriet D. Gliddon, et al.
Published: (2021-12-01)

New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA
by: Denis Sergeevich Naberezhnov, et al.
Published: (2023-09-01)

Application of genetic and evolutionary approaches in marine conservation and management (Case study: PANGAS project)
by: TOIC Katarina
Published: (2016-12-01)

Reconstructing each cell’s genome within complex microbial communities - dream or reality?
by: Scott eClingenpeel, et al.
Published: (2015-01-01)

RESEARCH AND DEVELOPMENT CONTROL METHOD PATHOGENIC PRION INFECTIONS SECONDARY RAW MEAT INDUSTRY
by: A. Y. Prosekov, et al.
Published: (2016-10-01)

An Emulsion System Based on a Chip Polymerase Chain Reaction
by: Pinfei Yu, et al.
Published: (2008-12-01)

Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins
by: Ken-ichi eLee, et al.
Published: (2015-02-01)

Plastome Rearrangements in the “Adenocalymma-Neojobertia” Clade (Bignonieae, Bignoniaceae) and Its Phylogenetic Implications
by: Luiz H. M. Fonseca, et al.
Published: (2017-11-01)

Characterization of whole genome amplified (WGA) DNA for use in genotyping assay development
by: Han Tao, et al.
Published: (2012-06-01)

Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators
by: Sarah V. Emser, et al.
Published: (2021-12-01)

Newcastle Disease Genotype VII Prevalence in Poultry and Wild Birds in Egypt
by: Amal A. M. Eid, et al.
Published: (2022-10-01)

Rapid whole genome sequencing methods for RNA viruses
by: Masayasu Misu, et al.
Published: (2023-02-01)

Keragaman Morfologi, Genetika, dan Patogenisitas Colletotrichum acutatum PenyebabAntraknosa Cabai di Jawa dan Sumatera
by: Roy Ibrahim, et al.
Published: (2017-05-01)

In vitro Identification of CACNA1H in the 9th Exon of Childhood Absence Epilepsy (CAE) Patients Using BioEdit and ClustalW2
by: Asma Irshad, et al.
Published: (2021-08-01)

Genetic identification of the causative agent of Brucellosis
by: Yerkebulan Jakipov, et al.
Published: (2021-07-01)

Primer Design and Analysis for Detection of mecA gene
by: Armini Syamsidi, et al.
Published: (2021-06-01)

Recent advances and application of whole genome amplification in molecular diagnosis and medicine
by: Xiaoyu Wang, et al.
Published: (2022-03-01)

Drug resistance analysis by next generation sequencing in Leishmania
by: Philippe Leprohon, et al.
Published: (2015-04-01)

Picobirnaviruses: prevalence, genetic diversity, detection methods
by: A. Yu. Kashnikov, et al.
Published: (2020-10-01)

Rescue of <i>Mycobacterium bovis</i> DNA Obtained from Cultured Samples during Official Surveillance of Animal TB: Key Steps for Robust Whole Genome Sequence Data Generation
by: Daniela Pinto, et al.
Published: (2024-03-01)

Whole-genome surveillance identifies markers of Plasmodium falciparum drug resistance and novel genomic regions under selection in Mozambique
by: Erin Coonahan, et al.
Published: (2023-10-01)