Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli in to active tetrameric protein
A tetrameric protein of therapeutic importance, Escherichia coli L-Asparaginase-II was expressed in Escherichia coli as inclusion bodies. Asparaginase inclusion bodies were solubilized using low concentration of urea and refolded into active tetrameric protein using pulsatile dilution method. Refold...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00486/full |