Purification of primary human placental leukocytes to study maternal-fetal interactions

Purification of primary human placental leukocytes to study maternal-fetal interactions

Summary: Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the m...

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Bibliographic Details
Main Authors: Nadia M. Ikumi, Zachary Koenig, Shweta Mahajan, Clive M. Gray, Tamara Tilburgs
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Cell separation/fractionation
Developmental biology
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002447

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http://www.sciencedirect.com/science/article/pii/S2666166723002447

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