α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials
<i>Celastrus hindsii</i> is a popular medicinal plant in Vietnam and Southeast Asian countries as well as in South America. In this study, an amount of 12.05 g of an α-amyrin and β-amyrin mixture was isolated from <i>C. hindsii</i> (10.75 g/kg dry weight) by column chromatogr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/23/7248 |
| _version_ | 1797507382532636672 |
|---|---|
| author | Tran Duc Viet Tran Dang Xuan La Hoang Anh |
| author_facet | Tran Duc Viet Tran Dang Xuan La Hoang Anh |
| author_sort | Tran Duc Viet |
| collection | DOAJ |
| description | <i>Celastrus hindsii</i> is a popular medicinal plant in Vietnam and Southeast Asian countries as well as in South America. In this study, an amount of 12.05 g of an α-amyrin and β-amyrin mixture was isolated from <i>C. hindsii</i> (10.75 g/kg dry weight) by column chromatography applying different solvent systems to obtain maximum efficiency. α-Amyrin and β-amyrin were then confirmed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR). The antioxidant activities of the α-amyrin and β-amyrin mixture were determined via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays with IC<sub>50</sub> of 125.55 and 155.28 µg/mL, respectively. The mixture exhibited a high potential for preventing gout by inhibiting a relevant key enzyme, xanthine oxidase (XO) (IC<sub>50</sub> = 258.22 µg/mL). Additionally, an important enzyme in skin hyperpigmentation, tyrosinase, was suppressed by the α-amyrin and β-amyrin mixture (IC<sub>50</sub> = 178.85 µg/mL). This study showed that <i>C. hindsii</i> is an abundant source for the isolation of α-amyrin and β-amyrin. Furthermore, this was the first study indicating that α-amyrin and β-amyrin mixture are promising in future therapies for gout and skin hyperpigmentation. |
| first_indexed | 2024-03-10T04:47:40Z |
| format | Article |
| id | doaj.art-b98e5aee06084693a767eef46f51a1f1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T04:47:40Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b98e5aee06084693a767eef46f51a1f12023-11-23T02:49:40ZengMDPI AGMolecules1420-30492021-11-012623724810.3390/molecules26237248α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase PotentialsTran Duc Viet0Tran Dang Xuan1La Hoang Anh2Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, JapanGraduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, JapanGraduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan<i>Celastrus hindsii</i> is a popular medicinal plant in Vietnam and Southeast Asian countries as well as in South America. In this study, an amount of 12.05 g of an α-amyrin and β-amyrin mixture was isolated from <i>C. hindsii</i> (10.75 g/kg dry weight) by column chromatography applying different solvent systems to obtain maximum efficiency. α-Amyrin and β-amyrin were then confirmed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR). The antioxidant activities of the α-amyrin and β-amyrin mixture were determined via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays with IC<sub>50</sub> of 125.55 and 155.28 µg/mL, respectively. The mixture exhibited a high potential for preventing gout by inhibiting a relevant key enzyme, xanthine oxidase (XO) (IC<sub>50</sub> = 258.22 µg/mL). Additionally, an important enzyme in skin hyperpigmentation, tyrosinase, was suppressed by the α-amyrin and β-amyrin mixture (IC<sub>50</sub> = 178.85 µg/mL). This study showed that <i>C. hindsii</i> is an abundant source for the isolation of α-amyrin and β-amyrin. Furthermore, this was the first study indicating that α-amyrin and β-amyrin mixture are promising in future therapies for gout and skin hyperpigmentation.https://www.mdpi.com/1420-3049/26/23/7248α-amyrinβ-amyrinGC-MSESI-MSNMRchromatography |
| spellingShingle | Tran Duc Viet Tran Dang Xuan La Hoang Anh α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials Molecules α-amyrin β-amyrin GC-MS ESI-MS NMR chromatography |
| title | α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials |
| title_full | α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials |
| title_fullStr | α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials |
| title_full_unstemmed | α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials |
| title_short | α-Amyrin and β-Amyrin Isolated from <i>Celastrus hindsii</i> Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials |
| title_sort | α amyrin and β amyrin isolated from i celastrus hindsii i leaves and their antioxidant anti xanthine oxidase and anti tyrosinase potentials |
| topic | α-amyrin β-amyrin GC-MS ESI-MS NMR chromatography |
| url | https://www.mdpi.com/1420-3049/26/23/7248 |
| work_keys_str_mv | AT tranducviet aamyrinandbamyrinisolatedfromicelastrushindsiiileavesandtheirantioxidantantixanthineoxidaseandantityrosinasepotentials AT trandangxuan aamyrinandbamyrinisolatedfromicelastrushindsiiileavesandtheirantioxidantantixanthineoxidaseandantityrosinasepotentials AT lahoanganh aamyrinandbamyrinisolatedfromicelastrushindsiiileavesandtheirantioxidantantixanthineoxidaseandantityrosinasepotentials |