An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for gene functional characterization and CRISPR/Cas9 genome...

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Bibliographic Details
Main Authors: Wenhui Yang, Jiaqin Ren, Wanrong Liu, Dan Liu, Kaidong Xie, Fei Zhang, Pengwei Wang, Wenwu Guo, Xiaomeng Wu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2023-06-01
Series:Horticultural Plant Journal
Subjects:
Citrus
Callus protoplast
Transient transfection
Subcellular localization
Genome editing
Online Access:http://www.sciencedirect.com/science/article/pii/S2468014122000590

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http://www.sciencedirect.com/science/article/pii/S2468014122000590

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