Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium
This study explores the synergistic antibacterial effects of essential oils (EOs) and phenolic extracts from three plants against foodborne pathogenic bacteria. The present work aimed to investigate the synergistic effects of the binary and the ternary combinations of extracts using different blend...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-02-01
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| Series: | Biomolecular Concepts |
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| Online Access: | https://doi.org/10.1515/bmc-2022-0040 |
| _version_ | 1797303268867571712 |
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| author | Benamar-Aissa Boualem Gourine Nadhir Ouinten Mohamed Yousfi Mohamed |
| author_facet | Benamar-Aissa Boualem Gourine Nadhir Ouinten Mohamed Yousfi Mohamed |
| author_sort | Benamar-Aissa Boualem |
| collection | DOAJ |
| description | This study explores the synergistic antibacterial effects of essential oils (EOs) and phenolic extracts from three plants against foodborne pathogenic bacteria. The present work aimed to investigate the synergistic effects of the binary and the ternary combinations of extracts using different blend proportions of the following plant extracts: Artemisia campestris (AC), Artemisia herba alba (AHA), and Citrus aurantium (CA). The antimicrobial activities of EOs and phenolic extracts were determined and evaluated against five strains. For the EOs, the results of the DIZ showed the existence of synergism for different combinations of binary blends, such as AC/AHA or AHA/CA against Escherichia coli, and AC/CA against Enterobacter faecalis. In addition, ternary blends of AC:AHA:CA at a ratio of 1/6:2/3:1/6 exhibited a synergy effect, as measured by the CI, against E. coli. On the other hand, for the phenolic extracts, synergistic effects were noticed for binary blends of AC/CA at different ratios against E. coli, E. faecalis, and Pseudomonas aeruginosa strains. Similarly, ternary blends of phenolic extracts presented synergy against E. coli, E. faecalis, P. aeruginosa strains, and even C. albicans. In this case, the blending ratios were crucial determining factors for maximizing the synergy effect. The study established that the proportion of a single drug could play an essential role in determining the bioefficacy of a drug combination treatment. Therefore, the results showed the importance of studying the modulation of antibacterial activities based on the proportions of extracts in the mixture and finding the range of proportions (as determined by SLMD) that have a synergistic/additive/antagonistic effect with no or low side effects, which can be used in a food preservation system. |
| first_indexed | 2024-03-07T23:49:27Z |
| format | Article |
| id | doaj.art-b9a00330d8df4465879d7b87b82f5d39 |
| institution | Directory Open Access Journal |
| issn | 1868-503X |
| language | English |
| last_indexed | 2024-03-07T23:49:27Z |
| publishDate | 2024-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Biomolecular Concepts |
| spelling | doaj.art-b9a00330d8df4465879d7b87b82f5d392024-02-19T09:01:21ZengDe GruyterBiomolecular Concepts1868-503X2024-02-01151e16804400610.1515/bmc-2022-0040Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantiumBenamar-Aissa Boualem0Gourine Nadhir1Ouinten Mohamed2Yousfi Mohamed3Laboratoire des Sciences Fondamentales (LSF), University Amar Telidji of Laghouat, BP. 37G (03000), Laghouat, AlgeriaLaboratoire des Sciences Fondamentales (LSF), University Amar Telidji of Laghouat, BP. 37G (03000), Laghouat, AlgeriaLaboratoire des Sciences Fondamentales (LSF), University Amar Telidji of Laghouat, BP. 37G (03000), Laghouat, AlgeriaLaboratoire des Sciences Fondamentales (LSF), University Amar Telidji of Laghouat, BP. 37G (03000), Laghouat, AlgeriaThis study explores the synergistic antibacterial effects of essential oils (EOs) and phenolic extracts from three plants against foodborne pathogenic bacteria. The present work aimed to investigate the synergistic effects of the binary and the ternary combinations of extracts using different blend proportions of the following plant extracts: Artemisia campestris (AC), Artemisia herba alba (AHA), and Citrus aurantium (CA). The antimicrobial activities of EOs and phenolic extracts were determined and evaluated against five strains. For the EOs, the results of the DIZ showed the existence of synergism for different combinations of binary blends, such as AC/AHA or AHA/CA against Escherichia coli, and AC/CA against Enterobacter faecalis. In addition, ternary blends of AC:AHA:CA at a ratio of 1/6:2/3:1/6 exhibited a synergy effect, as measured by the CI, against E. coli. On the other hand, for the phenolic extracts, synergistic effects were noticed for binary blends of AC/CA at different ratios against E. coli, E. faecalis, and Pseudomonas aeruginosa strains. Similarly, ternary blends of phenolic extracts presented synergy against E. coli, E. faecalis, P. aeruginosa strains, and even C. albicans. In this case, the blending ratios were crucial determining factors for maximizing the synergy effect. The study established that the proportion of a single drug could play an essential role in determining the bioefficacy of a drug combination treatment. Therefore, the results showed the importance of studying the modulation of antibacterial activities based on the proportions of extracts in the mixture and finding the range of proportions (as determined by SLMD) that have a synergistic/additive/antagonistic effect with no or low side effects, which can be used in a food preservation system.https://doi.org/10.1515/bmc-2022-0040essential oilsphenolic extractsantimicrobialsynergisticsimplex lattice mixture design (slmd) |
| spellingShingle | Benamar-Aissa Boualem Gourine Nadhir Ouinten Mohamed Yousfi Mohamed Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium Biomolecular Concepts essential oils phenolic extracts antimicrobial synergistic simplex lattice mixture design (slmd) |
| title | Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium |
| title_full | Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium |
| title_fullStr | Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium |
| title_full_unstemmed | Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium |
| title_short | Synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of Artemisia campestris, Artemisia herba alba, and Citrus aurantium |
| title_sort | synergistic effects of essential oils and phenolic extracts on antimicrobial activities using blends of artemisia campestris artemisia herba alba and citrus aurantium |
| topic | essential oils phenolic extracts antimicrobial synergistic simplex lattice mixture design (slmd) |
| url | https://doi.org/10.1515/bmc-2022-0040 |
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