High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation.

High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation.

A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J) regions were amplified from the spleen and lymph tissue of mice immunized with the human complement protein C5, fuse...

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Bibliographic Details
Main Authors: Audrey D McConnell, Minjee Do, Tamlyn Y Neben, Vladimir Spasojevic, Josh MacLaren, Andy P Chen, Laurence Altobell, John L Macomber, Ashley D Berkebile, Robert A Horlick, Peter M Bowers, David J King
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3498135?pdf=render

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http://europepmc.org/articles/PMC3498135?pdf=render

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