Optimized protocol for CRISPR knockout of human iPSC-derived macrophages

Optimized protocol for CRISPR knockout of human iPSC-derived macrophages

Summary: Here, we present a protocol for lentiviral delivery of CRISPR-Cas9 to human induced pluripotent stem cell (iPSC)-derived macrophages using co-incubation with VPX virus-like particles (VPX-VLPs). We describe steps for producing polybrene and puromycin kill curves, VPX viral production, and V...

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Bibliographic Details
Main Authors: Elena Navarro-Guerrero, Roberta Baronio, Chwen Tay, Julian C. Knight, Daniel V. Ebner
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
High Throughput Screening
Microbiology
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000686

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http://www.sciencedirect.com/science/article/pii/S2666166724000686

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