CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy

CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy

T cells engineered with chimeric antigen receptors (CARs) show great promise in the treatment of some cancers. Modifying T cells to express CARs generally relies on T-cell transduction using viral vectors carrying a transgene, resulting in semi-random DNA integration within the T-cell genome. While...

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Bibliographic Details
Main Authors: Zelda Odé, Jose Condori, Nicolas Peterson, Sheng Zhou, Giedre Krenciute
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Cancers
Subjects:
CAR
T cell
non-viral
HDR
CRISPR-Cas9
knock-in
Online Access:https://www.mdpi.com/2072-6694/12/6/1704

Internet

https://www.mdpi.com/2072-6694/12/6/1704

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