Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7
The effects of tuna cooking juice hydrolysates by protease XXIII (PA) on cell growth and induction of apoptosis of MCF-7 cell line were determined. The PA hydrolysates showed antiproliferative activities up to 25% against MCF-7 cells, and the >2.5 kDa ultrafiltration fraction (PAH2.5) possessed t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614002746 |
| _version_ | 1818937053954441216 |
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| author | Chuan-Chuan Hung Yu-Hsuan Yang Pei-Feng Kuo Kuo-Chiang Hsu |
| author_facet | Chuan-Chuan Hung Yu-Hsuan Yang Pei-Feng Kuo Kuo-Chiang Hsu |
| author_sort | Chuan-Chuan Hung |
| collection | DOAJ |
| description | The effects of tuna cooking juice hydrolysates by protease XXIII (PA) on cell growth and induction of apoptosis of MCF-7 cell line were determined. The PA hydrolysates showed antiproliferative activities up to 25% against MCF-7 cells, and the >2.5 kDa ultrafiltration fraction (PAH2.5) possessed the highest antiproliferative activity with an IC50 value of 1.39 mg/mL. PAH2.5 induced cell cycle arrest in S phase through the increases of p21 and p27, and decrease of cyclin A expression. Further, PAH2.5 also induced apoptosis of MCF-7 cells by downregulation of the expression of Bcl-2, PARP and caspase 9, and upregulation of the expression of p53, Bax and cleaved caspase 3. Two peptides were identified in PAH2.5 as KPEGMDPPLSEPEDRRDGAAGPK (2449.292 Da) and KLPPLLLAKLLMSGKLLAEPCTGR (2562.405 Da). Thus, tuna cooking juice may be a good protein source of antiproliferative peptides against MCF-7. |
| first_indexed | 2024-12-20T05:45:51Z |
| format | Article |
| id | doaj.art-ba009c2d3dd94f64aa753d0586122e95 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T05:45:51Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ba009c2d3dd94f64aa753d0586122e952022-12-21T19:51:20ZengElsevierJournal of Functional Foods1756-46462014-11-0111563570Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7Chuan-Chuan Hung0Yu-Hsuan Yang1Pei-Feng Kuo2Kuo-Chiang Hsu3Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, No. 500, Lioufeng Road, Taichung 41354, TaiwanDepartment of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, TaiwanDepartment of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, TaiwanDepartment of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, No. 500, Lioufeng Road, Taichung 41354, Taiwan; Corresponding author. Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. Tel.: +886 4 22053366 ext 7522; fax: +886 4 22062891.The effects of tuna cooking juice hydrolysates by protease XXIII (PA) on cell growth and induction of apoptosis of MCF-7 cell line were determined. The PA hydrolysates showed antiproliferative activities up to 25% against MCF-7 cells, and the >2.5 kDa ultrafiltration fraction (PAH2.5) possessed the highest antiproliferative activity with an IC50 value of 1.39 mg/mL. PAH2.5 induced cell cycle arrest in S phase through the increases of p21 and p27, and decrease of cyclin A expression. Further, PAH2.5 also induced apoptosis of MCF-7 cells by downregulation of the expression of Bcl-2, PARP and caspase 9, and upregulation of the expression of p53, Bax and cleaved caspase 3. Two peptides were identified in PAH2.5 as KPEGMDPPLSEPEDRRDGAAGPK (2449.292 Da) and KLPPLLLAKLLMSGKLLAEPCTGR (2562.405 Da). Thus, tuna cooking juice may be a good protein source of antiproliferative peptides against MCF-7.http://www.sciencedirect.com/science/article/pii/S1756464614002746Tuna cooking juicePeptidesBreast cancerAntiproliferationCell cycleApoptosis |
| spellingShingle | Chuan-Chuan Hung Yu-Hsuan Yang Pei-Feng Kuo Kuo-Chiang Hsu Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 Journal of Functional Foods Tuna cooking juice Peptides Breast cancer Antiproliferation Cell cycle Apoptosis |
| title | Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 |
| title_full | Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 |
| title_fullStr | Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 |
| title_full_unstemmed | Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 |
| title_short | Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7 |
| title_sort | protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line mcf 7 |
| topic | Tuna cooking juice Peptides Breast cancer Antiproliferation Cell cycle Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614002746 |
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