Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain
In Brazil, sucrose-rich broths (cane juice and/or molasses) are used to produce billions of liters of both fuel ethanol and <i>cachaça</i> per year using selected <i>Saccharomyces cerevisiae</i> industrial strains. Considering the important role of feedstock (sugar) prices in...
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| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/8/803 |
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| author | Gabriela Muller Victor R. de Godoy Marcelo G. Dário Eduarda H. Duval Sergio L. Alves-Jr Augusto Bücker Carlos A. Rosa Barbara Dunn Gavin Sherlock Boris U. Stambuk |
| author_facet | Gabriela Muller Victor R. de Godoy Marcelo G. Dário Eduarda H. Duval Sergio L. Alves-Jr Augusto Bücker Carlos A. Rosa Barbara Dunn Gavin Sherlock Boris U. Stambuk |
| author_sort | Gabriela Muller |
| collection | DOAJ |
| description | In Brazil, sucrose-rich broths (cane juice and/or molasses) are used to produce billions of liters of both fuel ethanol and <i>cachaça</i> per year using selected <i>Saccharomyces cerevisiae</i> industrial strains. Considering the important role of feedstock (sugar) prices in the overall process economics, to improve sucrose fermentation the genetic characteristics of a group of eight fuel-ethanol and five <i>cachaça</i> industrial yeasts that tend to dominate the fermentors during the production season were determined by array comparative genomic hybridization. The widespread presence of genes encoding invertase at multiple telomeres has been shown to be a common feature of both baker’s and distillers’ yeast strains, and is postulated to be an adaptation to sucrose-rich broths. Our results show that only two strains (one fuel-ethanol and one <i>cachaça</i> yeast) have amplification of genes encoding invertase, with high specific activity. The other industrial yeast strains had a single locus (<i>SUC2</i>) in their genome, with different patterns of invertase activity. These results indicate that invertase activity probably does not limit sucrose fermentation during fuel-ethanol and <i>cachaça</i> production by these industrial strains. Using this knowledge, we changed the mode of sucrose metabolism of an industrial strain by avoiding extracellular invertase activity, overexpressing the intracellular invertase, and increasing its transport through the <i>AGT1</i> permease. This approach allowed the direct consumption of the disaccharide by the cells, without releasing glucose or fructose into the medium, and a 11% higher ethanol production from sucrose by the modified industrial yeast, when compared to its parental strain. |
| first_indexed | 2024-03-10T23:49:48Z |
| format | Article |
| id | doaj.art-ba0e2d713f07448b9f9d16189166128d |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T23:49:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ba0e2d713f07448b9f9d16189166128d2023-11-19T01:47:14ZengMDPI AGJournal of Fungi2309-608X2023-07-019880310.3390/jof9080803Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast StrainGabriela Muller0Victor R. de Godoy1Marcelo G. Dário2Eduarda H. Duval3Sergio L. Alves-Jr4Augusto Bücker5Carlos A. Rosa6Barbara Dunn7Gavin Sherlock8Boris U. Stambuk9Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, BrazilDepartment of Genetics, Stanford University, Stanford, CA 94305, USADepartment of Genetics, Stanford University, Stanford, CA 94305, USADepartment of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina 88040-900, BrazilIn Brazil, sucrose-rich broths (cane juice and/or molasses) are used to produce billions of liters of both fuel ethanol and <i>cachaça</i> per year using selected <i>Saccharomyces cerevisiae</i> industrial strains. Considering the important role of feedstock (sugar) prices in the overall process economics, to improve sucrose fermentation the genetic characteristics of a group of eight fuel-ethanol and five <i>cachaça</i> industrial yeasts that tend to dominate the fermentors during the production season were determined by array comparative genomic hybridization. The widespread presence of genes encoding invertase at multiple telomeres has been shown to be a common feature of both baker’s and distillers’ yeast strains, and is postulated to be an adaptation to sucrose-rich broths. Our results show that only two strains (one fuel-ethanol and one <i>cachaça</i> yeast) have amplification of genes encoding invertase, with high specific activity. The other industrial yeast strains had a single locus (<i>SUC2</i>) in their genome, with different patterns of invertase activity. These results indicate that invertase activity probably does not limit sucrose fermentation during fuel-ethanol and <i>cachaça</i> production by these industrial strains. Using this knowledge, we changed the mode of sucrose metabolism of an industrial strain by avoiding extracellular invertase activity, overexpressing the intracellular invertase, and increasing its transport through the <i>AGT1</i> permease. This approach allowed the direct consumption of the disaccharide by the cells, without releasing glucose or fructose into the medium, and a 11% higher ethanol production from sucrose by the modified industrial yeast, when compared to its parental strain.https://www.mdpi.com/2309-608X/9/8/803bioethanolsugarcaneyeastfermentation |
| spellingShingle | Gabriela Muller Victor R. de Godoy Marcelo G. Dário Eduarda H. Duval Sergio L. Alves-Jr Augusto Bücker Carlos A. Rosa Barbara Dunn Gavin Sherlock Boris U. Stambuk Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain Journal of Fungi bioethanol sugarcane yeast fermentation |
| title | Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain |
| title_full | Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain |
| title_fullStr | Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain |
| title_full_unstemmed | Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain |
| title_short | Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain |
| title_sort | improved sugarcane based fermentation processes by an industrial fuel ethanol yeast strain |
| topic | bioethanol sugarcane yeast fermentation |
| url | https://www.mdpi.com/2309-608X/9/8/803 |
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