Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice
In this study, we investigated the effects of Ganoderma lucidum aqueous extract (GLW) on improving learning and memory impairment (LMI) and analyzed the key genes and pathways involved. GLW can improve LMI in mice by increasing the activities of SOD and GSH-Px, increasing the contents of GLU and ACH...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622003929 |
| _version_ | 1811179083468374016 |
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| author | Chunlei Ji Yue Yang Yujuan Fu Xinyi Pu Guangyu Xu |
| author_facet | Chunlei Ji Yue Yang Yujuan Fu Xinyi Pu Guangyu Xu |
| author_sort | Chunlei Ji |
| collection | DOAJ |
| description | In this study, we investigated the effects of Ganoderma lucidum aqueous extract (GLW) on improving learning and memory impairment (LMI) and analyzed the key genes and pathways involved. GLW can improve LMI in mice by increasing the activities of SOD and GSH-Px, increasing the contents of GLU and ACH, and reducing the contents of MDA and GABA. 644 differential expressed genes were screened by mRNA sequencing technology, and KEGG pathway analysis showed that Cholinergic synapse pathway was an important pathway for GLW to improve LMI in mice. Gnb1 and Adcy2 genes are the key genes in GLW pathway to improve LMI in mice. Gnb1 gene is positively regulated by M2 protein, which inhibits Cav-1 protein and promotes MAPK pathway. Adcy2 gene is promoted by nAChR protein and inhibited by Gnb1 gene, but it itself plays a positive regulatory role in CAMP pathway. |
| first_indexed | 2024-04-11T06:28:34Z |
| format | Article |
| id | doaj.art-ba2d085971a04c3393e7c8a27f544221 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T06:28:34Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ba2d085971a04c3393e7c8a27f5442212022-12-22T04:40:15ZengElsevierJournal of Functional Foods1756-46462022-12-0199105322Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging miceChunlei Ji0Yue Yang1Yujuan Fu2Xinyi Pu3Guangyu Xu4College of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, ChinaCollege of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, ChinaCollege of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, ChinaCollege of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, ChinaCorresponding author at: College of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, China.; College of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin 132013, ChinaIn this study, we investigated the effects of Ganoderma lucidum aqueous extract (GLW) on improving learning and memory impairment (LMI) and analyzed the key genes and pathways involved. GLW can improve LMI in mice by increasing the activities of SOD and GSH-Px, increasing the contents of GLU and ACH, and reducing the contents of MDA and GABA. 644 differential expressed genes were screened by mRNA sequencing technology, and KEGG pathway analysis showed that Cholinergic synapse pathway was an important pathway for GLW to improve LMI in mice. Gnb1 and Adcy2 genes are the key genes in GLW pathway to improve LMI in mice. Gnb1 gene is positively regulated by M2 protein, which inhibits Cav-1 protein and promotes MAPK pathway. Adcy2 gene is promoted by nAChR protein and inhibited by Gnb1 gene, but it itself plays a positive regulatory role in CAMP pathway.http://www.sciencedirect.com/science/article/pii/S1756464622003929Ganoderma lucidum water extractLearning and memory impairmentmRNA sequencingCholinergic Synapse PathwayMechanism of action |
| spellingShingle | Chunlei Ji Yue Yang Yujuan Fu Xinyi Pu Guangyu Xu Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice Journal of Functional Foods Ganoderma lucidum water extract Learning and memory impairment mRNA sequencing Cholinergic Synapse Pathway Mechanism of action |
| title | Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice |
| title_full | Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice |
| title_fullStr | Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice |
| title_full_unstemmed | Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice |
| title_short | Improvement of Ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d-galactose-induced aging mice |
| title_sort | improvement of ganoderma lucidum water extract on the learning and memory impairment and its mechanism in d galactose induced aging mice |
| topic | Ganoderma lucidum water extract Learning and memory impairment mRNA sequencing Cholinergic Synapse Pathway Mechanism of action |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622003929 |
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