Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans

Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans

Cell-free, chemoenzymatic platforms are emerging technologies towards generating glycoconjugates with defined and homogeneous glycoforms. Recombinant oligosaccharyltransferases can be applied to glycosylate “empty,” i.e., aglycosyalted, peptides and proteins. While bacterial oligosaccharlytransferas...

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Bibliographic Details
Main Authors: Lisa Wenzel, Marcus Hoffmann, Erdmann Rapp, Thomas F. T. Rexer, Udo Reichl
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-09-01
Series:Frontiers in Molecular Biosciences
Subjects:
multi-enzyme cascade reactions
in vitro N-glycosylation
synthetic glycobiotechnology
glycoengineering
oligosaccharyltransferase
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2023.1266431/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2023.1266431/full

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