Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs.

The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites. However, increasingly sophisticated experiments require increasingly complex DNA constructs, with...

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Bibliographic Details
Main Authors: Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3792108?pdf=render