The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use
Background: Lower limb ischemia-reperfusion injury (IRI-LL) is a common major complication of orthopedic surgery, especially in elderly patients. It has previously been demonstrated that folinic acid (FA) reduced IRI-LL damage in 3–4-month-old rats. This current work analyses the effect of FA in the...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1936 |
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| author | Borja Herrero de la Parte Javier Roa-Esparza Iñigo Cearra Inmaculada Ruiz Montesinos Daniel Alonso-Alconada Ana Alonso-Varona Carmen Mar Medina Sira Iturrizaga Correcher Ignacio García-Alonso |
| author_facet | Borja Herrero de la Parte Javier Roa-Esparza Iñigo Cearra Inmaculada Ruiz Montesinos Daniel Alonso-Alconada Ana Alonso-Varona Carmen Mar Medina Sira Iturrizaga Correcher Ignacio García-Alonso |
| author_sort | Borja Herrero de la Parte |
| collection | DOAJ |
| description | Background: Lower limb ischemia-reperfusion injury (IRI-LL) is a common major complication of orthopedic surgery, especially in elderly patients. It has previously been demonstrated that folinic acid (FA) reduced IRI-LL damage in 3–4-month-old rats. This current work analyses the effect of FA in the prevention of IRI-LL in elderly animals. Methods: Forty-two 18-month-old male WAG/RijHsd rats were subjected to 3 h of ischemia. Eighteen animals received FA (2.5 mg/kg, ip) 20 min before the end of the ischemia period, while the other half received the same volume of saline solution. The animals were sacrificed after 3 h, 24 h, and 14 days of reperfusion for biochemical (tissue damage markers and electrolytes), histopathological studies of the gastrocnemius muscle and the daily assessment of the limb function by the Rota Rod test, respectively. Results: The administration of FA prior to the end of the ischemia period reduced the increase in LDH and CK observed in non-treated animals by 30–40% (<i>p</i> < 0.0001). When the histological sections were analyzed, FA was found to have reduced the number of damaged muscle fibers per field by 20% (60 ± 17.1 vs. 80.7 ± 16.4, <i>p</i> < 0.0001). The functional test revealed that FA also led to an improvement in the muscle function, assessed by the length of time that the animals kept running on the rod, compared to untreated animals. Conclusions: The administration of FA, prior to the end of the ischemic period, decreases the damage induced by IRI-LL, also achieving a faster recovery of mobility. |
| first_indexed | 2024-03-09T20:49:50Z |
| format | Article |
| id | doaj.art-ba3a808579d8481894ee14b45829b889 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:50Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-ba3a808579d8481894ee14b45829b8892023-11-23T22:38:07ZengMDPI AGAntioxidants2076-39212022-09-011110193610.3390/antiox11101936The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet UseBorja Herrero de la Parte0Javier Roa-Esparza1Iñigo Cearra2Inmaculada Ruiz Montesinos3Daniel Alonso-Alconada4Ana Alonso-Varona5Carmen Mar Medina6Sira Iturrizaga Correcher7Ignacio García-Alonso8Department of Surgery and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Surgery and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Surgery and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Surgery and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainDepartment of Clinical Analyses, Galdakao-Usansolo Hospital, ES-48960 Galdakao, SpainDepartment of Clinical Analyses, Galdakao-Usansolo Hospital, ES-48960 Galdakao, SpainDepartment of Surgery and Radiology and Physical Medicine, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, ES-48940 Leioa, SpainBackground: Lower limb ischemia-reperfusion injury (IRI-LL) is a common major complication of orthopedic surgery, especially in elderly patients. It has previously been demonstrated that folinic acid (FA) reduced IRI-LL damage in 3–4-month-old rats. This current work analyses the effect of FA in the prevention of IRI-LL in elderly animals. Methods: Forty-two 18-month-old male WAG/RijHsd rats were subjected to 3 h of ischemia. Eighteen animals received FA (2.5 mg/kg, ip) 20 min before the end of the ischemia period, while the other half received the same volume of saline solution. The animals were sacrificed after 3 h, 24 h, and 14 days of reperfusion for biochemical (tissue damage markers and electrolytes), histopathological studies of the gastrocnemius muscle and the daily assessment of the limb function by the Rota Rod test, respectively. Results: The administration of FA prior to the end of the ischemia period reduced the increase in LDH and CK observed in non-treated animals by 30–40% (<i>p</i> < 0.0001). When the histological sections were analyzed, FA was found to have reduced the number of damaged muscle fibers per field by 20% (60 ± 17.1 vs. 80.7 ± 16.4, <i>p</i> < 0.0001). The functional test revealed that FA also led to an improvement in the muscle function, assessed by the length of time that the animals kept running on the rod, compared to untreated animals. Conclusions: The administration of FA, prior to the end of the ischemic period, decreases the damage induced by IRI-LL, also achieving a faster recovery of mobility.https://www.mdpi.com/2076-3921/11/10/1936ischemia-reperfusion injurylower limbelderly ratsprophylactic treatmentfolinic acidfunctional recovery |
| spellingShingle | Borja Herrero de la Parte Javier Roa-Esparza Iñigo Cearra Inmaculada Ruiz Montesinos Daniel Alonso-Alconada Ana Alonso-Varona Carmen Mar Medina Sira Iturrizaga Correcher Ignacio García-Alonso The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use Antioxidants ischemia-reperfusion injury lower limb elderly rats prophylactic treatment folinic acid functional recovery |
| title | The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use |
| title_full | The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use |
| title_fullStr | The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use |
| title_full_unstemmed | The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use |
| title_short | The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use |
| title_sort | prevention of ischemia reperfusion injury in elderly rats after lower limb tourniquet use |
| topic | ischemia-reperfusion injury lower limb elderly rats prophylactic treatment folinic acid functional recovery |
| url | https://www.mdpi.com/2076-3921/11/10/1936 |
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