Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe
The size and position of mitotic spindles is determined by the lengths of their constituent microtubules. Regulation of microtubule length requires feedback to set the balance between growth and shrinkage. Whereas negative feedback mechanisms for microtubule length control, based on depolymerizing k...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/10542 |
| _version_ | 1811200315534344192 |
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| author | Anneke Hibbel Aliona Bogdanova Mohammed Mahamdeh Anita Jannasch Marko Storch Erik Schäffer Dimitris Liakopoulos Jonathon Howard |
| author_facet | Anneke Hibbel Aliona Bogdanova Mohammed Mahamdeh Anita Jannasch Marko Storch Erik Schäffer Dimitris Liakopoulos Jonathon Howard |
| author_sort | Anneke Hibbel |
| collection | DOAJ |
| description | The size and position of mitotic spindles is determined by the lengths of their constituent microtubules. Regulation of microtubule length requires feedback to set the balance between growth and shrinkage. Whereas negative feedback mechanisms for microtubule length control, based on depolymerizing kinesins and severing proteins, have been studied extensively, positive feedback mechanisms are not known. Here, we report that the budding yeast kinesin Kip2 is a microtubule polymerase and catastrophe inhibitor in vitro that uses its processive motor activity as part of a feedback loop to further promote microtubule growth. Positive feedback arises because longer microtubules bind more motors, which walk to the ends where they reinforce growth and inhibit catastrophe. We propose that positive feedback, common in biochemical pathways to switch between signaling states, can also be used in a mechanical signaling pathway to switch between structural states, in this case between short and long polymers. |
| first_indexed | 2024-04-12T02:02:41Z |
| format | Article |
| id | doaj.art-ba4c6ae3190241f79a1e98a283b088f4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:02:41Z |
| publishDate | 2015-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ba4c6ae3190241f79a1e98a283b088f42022-12-22T03:52:38ZengeLife Sciences Publications LtdeLife2050-084X2015-11-01410.7554/eLife.10542Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastropheAnneke Hibbel0Aliona Bogdanova1Mohammed Mahamdeh2Anita Jannasch3Marko Storch4Erik Schäffer5Dimitris Liakopoulos6Jonathon Howard7Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Institute of Biochemistry, ETH Zurich, Zurich, SwitzerlandMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, GermanyDepartment of Molecular Biophysics & Biochemistry, Yale University, New Haven, United StatesMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Zentrum für Molekularbiologie der Pflanzen, Eberhard-Karls-Universität, Tübingen, GermanyDepartment of Life Sciences, Imperial College London, London, United KingdomZentrum für Molekularbiologie der Pflanzen, Eberhard-Karls-Universität, Tübingen, GermanyCRBM-CRNS, Montpellier, FranceDepartment of Molecular Biophysics & Biochemistry, Yale University, New Haven, United StatesThe size and position of mitotic spindles is determined by the lengths of their constituent microtubules. Regulation of microtubule length requires feedback to set the balance between growth and shrinkage. Whereas negative feedback mechanisms for microtubule length control, based on depolymerizing kinesins and severing proteins, have been studied extensively, positive feedback mechanisms are not known. Here, we report that the budding yeast kinesin Kip2 is a microtubule polymerase and catastrophe inhibitor in vitro that uses its processive motor activity as part of a feedback loop to further promote microtubule growth. Positive feedback arises because longer microtubules bind more motors, which walk to the ends where they reinforce growth and inhibit catastrophe. We propose that positive feedback, common in biochemical pathways to switch between signaling states, can also be used in a mechanical signaling pathway to switch between structural states, in this case between short and long polymers.https://elifesciences.org/articles/10542microtubule dynamicsmotor proteinlength regulationpositive feedback |
| spellingShingle | Anneke Hibbel Aliona Bogdanova Mohammed Mahamdeh Anita Jannasch Marko Storch Erik Schäffer Dimitris Liakopoulos Jonathon Howard Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe eLife microtubule dynamics motor protein length regulation positive feedback |
| title | Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe |
| title_full | Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe |
| title_fullStr | Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe |
| title_full_unstemmed | Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe |
| title_short | Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe |
| title_sort | kinesin kip2 enhances microtubule growth in vitro through length dependent feedback on polymerization and catastrophe |
| topic | microtubule dynamics motor protein length regulation positive feedback |
| url | https://elifesciences.org/articles/10542 |
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