Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9-based approach reveals high transcriptional variation

Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9-based approach reveals high transcriptional variation

Genomic safe harbors (GSH) are defined as sites in the host genome that allow stable expression of inserted transgenes while having no adverse effects on the host cell, making them ideal for use in basic research and therapeutic applications. Silencing and fluctuations in transgene expression would...

Full description

Bibliographic Details
Main Authors: Anne Inderbitzin, Tom Loosli, Roger D. Kouyos, Karin J. Metzner
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
CRISPR-Cas9
barcoded transgene
genomic safe harbor
AAVS1
Jurkat T cells
HIV-1 promoter
Online Access:http://www.sciencedirect.com/science/article/pii/S232905012200081X

Internet

http://www.sciencedirect.com/science/article/pii/S232905012200081X

Similar Items