Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells
In parthanatos, a PARP-1 (poly (ADP-ribose) polymerase 1)-mediated cell death, dissipation of mitochondrial membrane potential, large-scale DNA fragmentation and chromatin condensation were observed. In contrast to apoptosis, it does not cause apoptotic bodies formation. Although PARP-1-mediated cel...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2013-11-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2224 |
| _version_ | 1828471297858863104 |
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| author | P. Donizy A. Halon P. Surowiak G. Pietrzyk C. Kozyra R. Matkowski |
| author_facet | P. Donizy A. Halon P. Surowiak G. Pietrzyk C. Kozyra R. Matkowski |
| author_sort | P. Donizy |
| collection | DOAJ |
| description | In parthanatos, a PARP-1 (poly (ADP-ribose) polymerase 1)-mediated cell death, dissipation of mitochondrial membrane potential, large-scale DNA fragmentation and chromatin condensation were observed. In contrast to apoptosis, it does not cause apoptotic bodies formation. Although PARP-1-mediated cell death presents loss of membrane integrity similar to necrosis, it does not induce cell swelling. The purpose of the study was to correlate the immunohistochemical parameters of PARP-1 reactivity and the selected cytomorphological features of parthanatos: the lack of apoptotic bodies and the absence of necrosis in breast cancer (BC) specimens. Immunohistochemistry for PARP-1 was performed on 83 BC specimens. Correlations between parameters of PARP-1 expression and sub-cellular localisation and the presence of apoptotic bodies and necrosis were evaluated. High expression of PARP-1 (ImmunoReactive Score ≥6) was associated with the lack of apoptotic bodies (P=0.013) and with the absence of necrosis (P=0.002). The presence of apoptotic bodies was correlated with re-distribution of PARP-1 from the nucleus to cytoplasm in BC cells (P=0.029). Additionally, a tendency was observed between necrosis and loss of nuclear PARP-1 expression (P=0.049). Our study suggests that PARP-1 may play a crucial role in induction and regulation of specific type of cellular death called parthanatos. |
| first_indexed | 2024-12-11T05:09:53Z |
| format | Article |
| id | doaj.art-ba5c03ff564a48e8af033c3b7175f55e |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T05:09:53Z |
| publishDate | 2013-11-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-ba5c03ff564a48e8af033c3b7175f55e2022-12-22T01:19:57ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062013-11-01574e35e3510.4081/ejh.2013.e351370Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cellsP. Donizy0A. Halon1P. Surowiak2G. Pietrzyk3C. Kozyra4R. Matkowski5Wroclaw Medical UniversityWroclaw Medical UniversityWroclaw Medical University4th Military Hospital WroclawWroclaw University of EconomicsLower Silesian Oncology Centre WroclawIn parthanatos, a PARP-1 (poly (ADP-ribose) polymerase 1)-mediated cell death, dissipation of mitochondrial membrane potential, large-scale DNA fragmentation and chromatin condensation were observed. In contrast to apoptosis, it does not cause apoptotic bodies formation. Although PARP-1-mediated cell death presents loss of membrane integrity similar to necrosis, it does not induce cell swelling. The purpose of the study was to correlate the immunohistochemical parameters of PARP-1 reactivity and the selected cytomorphological features of parthanatos: the lack of apoptotic bodies and the absence of necrosis in breast cancer (BC) specimens. Immunohistochemistry for PARP-1 was performed on 83 BC specimens. Correlations between parameters of PARP-1 expression and sub-cellular localisation and the presence of apoptotic bodies and necrosis were evaluated. High expression of PARP-1 (ImmunoReactive Score ≥6) was associated with the lack of apoptotic bodies (P=0.013) and with the absence of necrosis (P=0.002). The presence of apoptotic bodies was correlated with re-distribution of PARP-1 from the nucleus to cytoplasm in BC cells (P=0.029). Additionally, a tendency was observed between necrosis and loss of nuclear PARP-1 expression (P=0.049). Our study suggests that PARP-1 may play a crucial role in induction and regulation of specific type of cellular death called parthanatos.http://www.ejh.it/index.php/ejh/article/view/2224parthanatos, cell death, PARP-1, immunohistochemistry, breast cancer. |
| spellingShingle | P. Donizy A. Halon P. Surowiak G. Pietrzyk C. Kozyra R. Matkowski Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells European Journal of Histochemistry parthanatos, cell death, PARP-1, immunohistochemistry, breast cancer. |
| title | Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells |
| title_full | Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells |
| title_fullStr | Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells |
| title_full_unstemmed | Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells |
| title_short | Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells |
| title_sort | correlation between parp 1 immunoreactivity and cytomorphological features of parthanatos a specific cellular death in breast cancer cells |
| topic | parthanatos, cell death, PARP-1, immunohistochemistry, breast cancer. |
| url | http://www.ejh.it/index.php/ejh/article/view/2224 |
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