Efficient culturing and genetic manipulation of human pluripotent stem cells.

Human pluripotent stem cells (hPSC) hold great promise as models for understanding disease and as a source of cells for transplantation therapies. However, the lack of simple, robust and efficient culture methods remains a significant obstacle for realizing the utility of hPSCs. Here we describe a p...

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Bibliographic Details
Main Authors: Robert T Schinzel, Tim Ahfeldt, Frank H Lau, Youn-Kyoung Lee, Alicia Cowley, Tony Shen, Derek Peters, David H Lum, Chad A Cowan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3240614?pdf=render