High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain)
The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding, but the effects of dietary lipids (fat) on low salinity adaptations have not been well evaluated. In the present study, a total of 120 mud crabs (Scylla paramamosain, BW = 17...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-03-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654522001500 |
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| author | Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R. Tocher Min Jin |
| author_facet | Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R. Tocher Min Jin |
| author_sort | Jiaxiang Luo |
| collection | DOAJ |
| description | The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding, but the effects of dietary lipids (fat) on low salinity adaptations have not been well evaluated. In the present study, a total of 120 mud crabs (Scylla paramamosain, BW = 17.87 ± 1.49 g) were fed control and high-fat (HF) diets, at both medium salinity (23‰) and low salinity (4‰) for 6 wk, and each treatment had 3 replicates with each replicate containing 10 crabs. The results indicated that a HF diet significantly mitigated the reduction in survival rate, percent weight gain and feed efficiency induced by low salinity (P < 0.05). Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs (P < 0.05). Thus, HF diets enhanced the process of lipolysis to supply more energy. In the gills, low salinity and the HF diet increased the levels of mitochondrial biogenesis markers, the activity of mitochondrial complexes, and the expression levels of genes related to energy metabolism (P < 0.05). Consequently, the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure regulation. Specifically, significantly higher haemolymph osmotic pressure and inorganic ion content, as well as higher osmotic pressure regulatory enzyme activity in gills, and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity (P < 0.05). In summary, high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis, which increased ATP provision for osmotic pressure regulation of mud crabs. This study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs. |
| first_indexed | 2024-04-09T21:45:56Z |
| format | Article |
| id | doaj.art-ba8a80e3fa35480f883ae11c7fcc79c7 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-04-09T21:45:56Z |
| publishDate | 2023-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-ba8a80e3fa35480f883ae11c7fcc79c72023-03-25T05:11:54ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452023-03-0112297307High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain)Jiaxiang Luo0Chen Ren1Tingting Zhu2Chen Guo3Shichao Xie4Yingying Zhang5Zheng Yang6Wenli Zhao7Xiangsheng Zhang8Jingjing Lu9Lefei Jiao10Qicun Zhou11Douglas R. Tocher12Min Jin13Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Corresponding author.The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding, but the effects of dietary lipids (fat) on low salinity adaptations have not been well evaluated. In the present study, a total of 120 mud crabs (Scylla paramamosain, BW = 17.87 ± 1.49 g) were fed control and high-fat (HF) diets, at both medium salinity (23‰) and low salinity (4‰) for 6 wk, and each treatment had 3 replicates with each replicate containing 10 crabs. The results indicated that a HF diet significantly mitigated the reduction in survival rate, percent weight gain and feed efficiency induced by low salinity (P < 0.05). Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs (P < 0.05). Thus, HF diets enhanced the process of lipolysis to supply more energy. In the gills, low salinity and the HF diet increased the levels of mitochondrial biogenesis markers, the activity of mitochondrial complexes, and the expression levels of genes related to energy metabolism (P < 0.05). Consequently, the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure regulation. Specifically, significantly higher haemolymph osmotic pressure and inorganic ion content, as well as higher osmotic pressure regulatory enzyme activity in gills, and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity (P < 0.05). In summary, high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis, which increased ATP provision for osmotic pressure regulation of mud crabs. This study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs.http://www.sciencedirect.com/science/article/pii/S2405654522001500Osmotic pressure regulationMitochondrial energy metabolismLipid metabolism |
| spellingShingle | Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R. Tocher Min Jin High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) Animal Nutrition Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism |
| title | High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) |
| title_full | High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) |
| title_fullStr | High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) |
| title_full_unstemmed | High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) |
| title_short | High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain) |
| title_sort | high dietary lipid level promotes low salinity adaptation in the marine euryhaline crab scylla paramamosain |
| topic | Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405654522001500 |
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