Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been dev...

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Bibliographic Details
Main Authors: José Carlos Pelielo de Mattos, Ellen Serri da Motta, Márcia Betania Nunes de Oliveira, Flávio José da Silva Dantas, Adriano Caldeira de Araujo
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2008-12-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Alkaline gel electrophoresis
Escherichia coli
base excision repair
stannous chloride
DNA strand breaks
reactive oxygen species
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020

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