T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells
Innate lymphoid cells (ILC) play a significant immunological role at mucosal surfaces such as the intestine. T-bet-expressing group 1 innate lymphoid cells (ILC1) are believed to play a substantial role in inflammatory bowel disease (IBD). However, a role of T-bet-negative ILC3 in driving colitis ha...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.623324/full |
| _version_ | 1818874983872462848 |
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| author | Jan-Hendrik Schroeder Katrin Meissl Dominika Hromadová Jonathan W. Lo Jonathan W. Lo Joana F. Neves Jane K. Howard Helena Helmby Nick Powell Birgit Strobl Graham M. Lord Graham M. Lord |
| author_facet | Jan-Hendrik Schroeder Katrin Meissl Dominika Hromadová Jonathan W. Lo Jonathan W. Lo Joana F. Neves Jane K. Howard Helena Helmby Nick Powell Birgit Strobl Graham M. Lord Graham M. Lord |
| author_sort | Jan-Hendrik Schroeder |
| collection | DOAJ |
| description | Innate lymphoid cells (ILC) play a significant immunological role at mucosal surfaces such as the intestine. T-bet-expressing group 1 innate lymphoid cells (ILC1) are believed to play a substantial role in inflammatory bowel disease (IBD). However, a role of T-bet-negative ILC3 in driving colitis has also been suggested in mouse models questioning T-bet as a critical factor for IBD. We report here that T-bet deficient mice had a greater cellularity of NKp46-negative ILC3 correlating with enhanced expression of RORγt and IL-7R, but independent of signaling through STAT1 or STAT4. We observed enhanced neutrophilia in the colonic lamina propria (cLP) of these animals, however, we did not detect a greater risk of T-bet-deficient mice to develop spontaneous colitis. Furthermore, by utilizing an in vivo fate-mapping approach, we identified a population of T-bet-positive precursors in NKp46-negative ILC3s. These data suggest that T-bet controls ILC3 cellularity, but does do not drive a pathogenic role of ILC3 in mice with a conventional specific pathogen-free microbiota. |
| first_indexed | 2024-12-19T13:19:16Z |
| format | Article |
| id | doaj.art-ba9f9bb4005040f781058400f748fa39 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T13:19:16Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ba9f9bb4005040f781058400f748fa392022-12-21T20:19:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-02-011110.3389/fimmu.2020.623324623324T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid CellsJan-Hendrik Schroeder0Katrin Meissl1Dominika Hromadová2Jonathan W. Lo3Jonathan W. Lo4Joana F. Neves5Jane K. Howard6Helena Helmby7Nick Powell8Birgit Strobl9Graham M. Lord10Graham M. Lord11School of Immunology and Microbial Sciences, King’s College London, London, United KingdomInstitute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, AustriaInstitute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, AustriaSchool of Immunology and Microbial Sciences, King’s College London, London, United KingdomDivision of Digestive Diseases, Faculty of Medicine, Imperial College London, London, United KingdomCentre for Host-Microbiome Interactions, King’s College London, London, United KingdomDepartment of Diabetes, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King’s College, London, United KingdomDepartment of Infection Biology, London School of Hygiene and Tropical Medicine, London, United KingdomDivision of Digestive Diseases, Faculty of Medicine, Imperial College London, London, United KingdomInstitute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, AustriaSchool of Immunology and Microbial Sciences, King’s College London, London, United KingdomFaculty of Biology, Medicine and Health, University of Manchester, Manchester, United KingdomInnate lymphoid cells (ILC) play a significant immunological role at mucosal surfaces such as the intestine. T-bet-expressing group 1 innate lymphoid cells (ILC1) are believed to play a substantial role in inflammatory bowel disease (IBD). However, a role of T-bet-negative ILC3 in driving colitis has also been suggested in mouse models questioning T-bet as a critical factor for IBD. We report here that T-bet deficient mice had a greater cellularity of NKp46-negative ILC3 correlating with enhanced expression of RORγt and IL-7R, but independent of signaling through STAT1 or STAT4. We observed enhanced neutrophilia in the colonic lamina propria (cLP) of these animals, however, we did not detect a greater risk of T-bet-deficient mice to develop spontaneous colitis. Furthermore, by utilizing an in vivo fate-mapping approach, we identified a population of T-bet-positive precursors in NKp46-negative ILC3s. These data suggest that T-bet controls ILC3 cellularity, but does do not drive a pathogenic role of ILC3 in mice with a conventional specific pathogen-free microbiota.https://www.frontiersin.org/articles/10.3389/fimmu.2020.623324/fullT-betinnate lymphoid cellsILCsintestinal inflammationmucosal homeostasis |
| spellingShingle | Jan-Hendrik Schroeder Katrin Meissl Dominika Hromadová Jonathan W. Lo Jonathan W. Lo Joana F. Neves Jane K. Howard Helena Helmby Nick Powell Birgit Strobl Graham M. Lord Graham M. Lord T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells Frontiers in Immunology T-bet innate lymphoid cells ILCs intestinal inflammation mucosal homeostasis |
| title | T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells |
| title_full | T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells |
| title_fullStr | T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells |
| title_full_unstemmed | T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells |
| title_short | T-Bet Controls Cellularity of Intestinal Group 3 Innate Lymphoid Cells |
| title_sort | t bet controls cellularity of intestinal group 3 innate lymphoid cells |
| topic | T-bet innate lymphoid cells ILCs intestinal inflammation mucosal homeostasis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.623324/full |
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