Continuous fluorescence assay for lecithin:cholesterol acyltransferase using a water-soluble phosphatidylcholine.
A water-soluble fluorescent phosphatidylcholine, 1,2-bis[4-(1-pyreno-butanoyl]-sn-glycero-3-phosphocholine (DPybPC) has been used to develop a sensitive, continuous assay for pure lecithin:cholesterol acyltransferase (LCAT) in solution. The monomeric substrate allowed us to examine the reaction of L...
| Main Authors: | , |
|---|---|
| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
Elsevier
1992-12-01
|
| Series: | Journal of Lipid Research |
| Acceso en liña: | http://www.sciencedirect.com/science/article/pii/S0022227520413446 |